Pan Li, Liu Yan, Lan Hainan, Bao Nan, Zhao Yuan, Sun Hui, Qin Guixin, Farouk Mohammed Hamdy
Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Animal Production Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
Front Vet Sci. 2021 Jun 2;8:639792. doi: 10.3389/fvets.2021.639792. eCollection 2021.
Soybean agglutinin (SBA) has a toxic effect on most animals. The anti-nutritional mechanisms of SBA are not fully understood, in terms of cell survival activity and metabolism of intestinal cells. This study aims to investigate the effects of SBA on the cell cycle, apoptosis, and to verify the mechanism of SBA anti-nutritional characters based on proteomic-based analysis. The IPEC-J2 cell line was cultured with medium containing 0.0, 0.5, or 2.0 mg/mL SBA. With increasing SBA levels, the percentage of the cells at G0/G1 phase, cell apoptosis rates, expressions of Bax and p21, and the activities of Casp-3 and Casp-9 were increased, while cyclin D1 and Bcl-2 expressions were declined ( < 0.05). The proteomic analysis showed that the numbers of differentially expressed proteins, induced by SBA, were mainly enriched in different pathways including DNA replication, base excision repair, nucleus excision repair, mismatch repair, amide and peptide biosynthesis, ubiquitin-mediated proteolysis, as well as structures and functions of mitochondria and ribosome. In conclusion, the anti-nutritional mechanism of SBA is a complex cellular process. Such process including DNA related activities; protein synthesis and metabolism; signal-conducting relation; as well as subcellular structure and function. This study provides comprehensive information to understand the toxic mechanism of SBA in monogastrics.
大豆凝集素（SBA）对大多数动物具有毒性作用。就肠道细胞的细胞存活活性和代谢而言，SBA的抗营养机制尚未完全明确。本研究旨在探讨SBA对细胞周期、细胞凋亡的影响，并基于蛋白质组学分析验证SBA抗营养特性的机制。将IPEC-J2细胞系培养于含有0.0、0.5或2.0 mg/mL SBA的培养基中。随着SBA水平的升高，处于G0/G1期的细胞百分比、细胞凋亡率、Bax和p21的表达以及Casp-3和Casp-9的活性均增加，而细胞周期蛋白D1和Bcl-2的表达则下降（P<0.05）。蛋白质组学分析表明，SBA诱导的差异表达蛋白数量主要富集于不同的途径，包括DNA复制、碱基切除修复、核苷酸切除修复、错配修复、酰胺和肽生物合成、泛素介导的蛋白水解以及线粒体和核糖体的结构与功能。总之，SBA的抗营养机制是一个复杂的细胞过程。该过程包括与DNA相关的活动；蛋白质合成与代谢；信号传导关系；以及亚细胞结构与功能。本研究为理解SBA在单胃动物中的毒性机制提供了全面的信息。
