Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Department of Food technology, College of Biochemical Engineering, Yangzhou Polytechnic College, Yangzhou, China.
Anim Biotechnol. 2023 Feb;34(1):56-66. doi: 10.1080/10495398.2021.1935981. Epub 2021 Jun 21.
Mono-Sex culturing is an important methodology for intensive livestock and poultry production. Here, was identified as a potential key gene in sex-determination process in chickens via RNA-seq. Then we developed an effective method to interfere or overexpress in chicken embryos through the intravascular injection. QRT-PCR, ELISA and H&E staining were used to detect the effects of on gonadal development of chicken embryos. Results showed that exhibited a female-biased expression pattern in the early stage of PGCs (primordial germ cells) in embryonic gonads. The qRT-PCR analysis showed that , in females were upregulated under the overexpression of , while and were downregulated . Overexpression of can promote the development of gonadal cortex, while interference with show the opposite result. Additionally, we found that overexpression of the in male chicken embryos could inhibit androgen levels and increase estrogen levels. On the other hand, interfering with in female chicken embryos decreased estrogen levels and increased androgen levels. In conclusion, this work sets the basis for the understanding of the molecular regulatory network for the sex-determination process in chicken embryos as well as providing the theoretical basis for mono-sex culturing of poultry.
单性繁殖是集约化畜牧业和家禽生产的重要方法。在这里，我们通过 RNA-seq 鉴定出在鸡的性别决定过程中一个潜在的关键基因 。然后，我们开发了一种通过血管内注射干扰或过表达 的有效方法。我们使用 QRT-PCR、ELISA 和 H&E 染色来检测 对鸡胚性腺发育的影响。结果表明， 在胚胎性腺的原始生殖细胞（PGCs）早期表现出雌性偏倚表达模式。qRT-PCR 分析表明， 在雌性中， 在 的过表达下上调，而 和 下调。过表达 可以促进性腺皮质的发育，而干扰 则显示出相反的结果。此外，我们发现，在雄性鸡胚中过表达 可以抑制雄激素水平并增加雌激素水平。另一方面，在雌性鸡胚中干扰 会降低雌激素水平并增加雄激素水平。总之，这项工作为理解鸡胚性别决定过程中的分子调控网络奠定了基础，并为家禽的单性繁殖提供了理论依据。
