Forest Department, Forestry College, Hebei Agricultural University, Baoding, China.
Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, 071000, People's Republic of China.
Plant Mol Biol. 2021 Aug;106(6):491-503. doi: 10.1007/s11103-021-01166-4. Epub 2021 Jun 24.
Anthocyanin was highly accumulated in the leaves of red-leaved poplars; Many structural genes involved in anthocyanin synthesis were significantly up-regulated in 'Quanhong' and 'Xuanhong'; TTG2, HYH, and HY5 may be directly involved in the regulation of anthocyanin synthesis in both red-leaved poplars. The red-leaved poplar cultivars 'Quanhong' and 'Xuanhong' are bud mutations of Populus deltoides cv. 'Zhonglin 2025'. These cultivars are valued for their beautiful shape, lack of flying catkins, and ornamental leaf colors. However, the understanding of the molecular mechanism of anthocyanin accumulation in the leaves of red-leaved poplars is still unclear. Here, we profiled the changes of pigment content, transcriptome and proteome expression in the leaves of three poplar cultivars and the results showed that the ratios of anthocyanin to total chlorophyll in both red-leaved poplars were higher than that in 'Zhonglin 2025', indicating that the anthocyanin was highly accumulated in the leaves of red-leaved poplars. Based on the results of combined transcriptome and proteome analysis, 15 and 11 differentially expressed genes/proteins involved in anthocyanin synthesis were screened in 'Quanhong' and 'Xuanhong', respectively, indicating that the two red-leaved poplar cultivars have slightly different patterns of regulating anthocyanin biosynthesis. Among the 120 transcription factors, 3 (HY5, HYH, and TTG2), may be directly involved in the regulation of anthocyanin synthesis in both red-leaved poplars. This study screens the candidate genes involved in anthocyanin accumulation in the leaves of red-leaved poplars and lays a foundation for further exploring the molecular mechanism of leaf red coloration in red-leaved poplars.
花色素苷在红叶杨叶片中高度积累；许多参与花色素苷合成的结构基因在‘全红’和‘炫红’中显著上调；TTG2、HYH 和 HY5 可能直接参与了这两个红叶杨中花色素苷合成的调节。红叶杨品种‘全红’和‘炫红’是中林 2025 杨的芽变。这些品种以其美丽的形状、无飞絮和观赏叶色而受到重视。然而，对红叶杨叶片花色素苷积累的分子机制的理解仍不清楚。在这里，我们对三个杨树种质叶片的色素含量、转录组和蛋白质组表达变化进行了分析，结果表明，两个红叶杨的叶片中花色素苷与总叶绿素的比例均高于中林 2025，表明花色素苷在红叶杨叶片中高度积累。基于转录组和蛋白质组联合分析的结果，在‘全红’和‘炫红’中分别筛选到 15 个和 11 个参与花色素苷合成的差异表达基因/蛋白，表明这两个红叶杨品种在调节花色素苷生物合成方面略有不同。在 120 个转录因子中，3 个（HY5、HYH 和 TTG2）可能直接参与了两个红叶杨中花色素苷合成的调节。本研究筛选了红叶杨叶片花色素苷积累相关的候选基因，为进一步探讨红叶杨叶片红叶形成的分子机制奠定了基础。
