在二倍体和四倍体棉花（棉属）中全基因组分析 IQD 基因家族。

Genome wide analysis of IQD gene family in diploid and tetraploid species of cotton (Gossypium spp.).

机构信息

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450000, China; State Key Laboratory of Cotton Biology, Institute of Cotton Research Chinese Academy of Agricultural Science, Anyang 455000, Henan, China.

State Key Laboratory of Cotton Biology, Institute of Cotton Research Chinese Academy of Agricultural Science, Anyang 455000, Henan, China.

出版信息

Int J Biol Macromol. 2021 Aug 1;184:1035-1061. doi: 10.1016/j.ijbiomac.2021.06.115. Epub 2021 Jun 24.

DOI:10.1016/j.ijbiomac.2021.06.115

PMID:34174315

Abstract

Calmodulin (CaM) is considered as the most significant Ca signaling messenger that mediate various biochemical and physiological reactions. IQ domain (IQD) proteins are plant specific CML/CaM calcium binding which are characterized by domains of 67 amino acids. 50, 50, 94, and 99 IQD genes were detected from G. arboreum (A2), G. raimondii (D5), G. barbadense (AD2) and G. hirsutum (AD1) respectively. Existence of more orthologous genes in cotton species than Arabidopsis, advocated that polyploidization produced new cotton specific orthologous gene clusters. Duplication of gene events depicts that IQD gene family of cotton evolution was under strong purifying selection. G. hirsutum exhibited high level synteny. GarIQD25 exhibited high expression in stem, root, flower, ovule and fiber in G. arboreum. In G. raimondii, GraIQD03 demonstrated upregulation across stem, ovule, fiber and seed. GbaIQD11 and GbaIQD62 exhibited upregulation in fiber development in G. barbadense. GhiIQD69 recognized as main candidate genes for plant parts, floral tissues, fiber and ovule development. Promotor analysis identified cis-regulatory elements were involved in plant growth and development. Overwhelmingly, present study paves the way to better understand the evolution of cotton IQD genes and lays a foundation for future investigation of IQD in cotton.

摘要

钙调蛋白（CaM）被认为是最重要的钙信号信使，介导各种生化和生理反应。IQ 结构域（IQD）蛋白是植物特异性的 CML/CaM 钙结合蛋白，其特征是由 67 个氨基酸组成的结构域。从 G. arboreum（A2）、G. raimondii（D5）、G. barbadense（AD2）和 G. hirsutum（AD1）中分别检测到 50、50、94 和 99 个 IQD 基因。在棉花物种中存在比拟南芥更多的同源基因，这表明多倍化产生了新的棉花特异性同源基因簇。基因事件的复制表明，棉花 IQD 基因家族的进化受到强烈的纯化选择。G. hirsutum 表现出高度的同线性。GarIQD25 在 G. arboreum 的茎、根、花、胚珠和纤维中表达水平较高。在 G. raimondii 中，GraIQD03 在茎、胚珠、纤维和种子中均上调表达。GbaIQD11 和 GbaIQD62 在 G. barbadense 的纤维发育中上调表达。GhiIQD69 被认为是植物部分、花组织、纤维和胚珠发育的主要候选基因。启动子分析确定了参与植物生长和发育的顺式调控元件。综上所述，本研究为更好地理解棉花 IQD 基因的进化铺平了道路，并为未来棉花 IQD 的研究奠定了基础。