Utility of Silver-nanoparticles for Nano Spectrofluorimetric Determination of Meropenem and Ertapenem: Bio-analytical Validation.

Bio-analytical, nano-quantitative spectrofluorimetric estimation of two non-classical β-lactam antibiotics; meropenem (MP) and ertapenem (EP) is presented. The method is based on the enhancement of the fluorescence intensity of MP-Eu/EP-Eu with silver nanoparticles (AgNPs). AgNPs were synthesized and characterized by UV and transmission electron microscope (TEM). The plasmon resonance produced an intense absorption maximum at 398.0 nm. TEM micrograph showed the particle morphology with an average particles size of 13.0 ± 2.95 nm. The fluorescence intensities were measured against blank reagents at λem of 396.0 nm and 405.0 nm after excitation at λex 305.0 nm and 303.0 nm for MP and EP, respectively. Under optimum conditions, the relative fluorescence intensity showed a good linear relationship with the concentration ranges of 4.0-14.0 and 4.0 -12.0 ng/mL with excellent correlation coefficients of 0.9998 and 0.9997, and limit of detection of 0.84 and 0.86 ng/mL for MP and EP, respectively. The method was successfully applied for direct analysis of MP and EP in their drug substances and pharmaceutical vials. The significant, sensitivity and practicality of the method facilitated MP detection in real plasma samples. Bio-analytical validation was performed according to FDA. The method was rectilinear over the ranges of, 5.0 -75.0 μg/mL plasma. Interestingly, this described system has a promising benefit for various applications exploiting the dramatically enhanced-fluorescence occurrence.