Malungo Illke B, Gravett Nadine, Bhagwandin Adhil, Davimes Joshua G, Manger Paul R
School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2193 Johannesburg, South Africa.
IBRO Neurosci Rep. 2021 Feb 17;10:142-152. doi: 10.1016/j.ibneur.2021.02.005. eCollection 2021 Jun.
Most studies examining sleep in mammals are done under controlled conditions in laboratory/zoological facilities with few studies being conducted in their natural environment. It is not always possible to record sleep polysomnographically (PSG) from animals in their natural environments, as PSG is invasive, requiring the surgical implantation of electrodes on the surface of the brain. In contrast, actigraphy (ACT) has been shown to be a minimally-invasive method to objectively measure overall sleep times in some mammals, although not revealing specific sleep states. The aim of this study is two-fold, first, to measure sleep polysomnographically in free-roaming blue wildebeest () under the most natural conditions possible, and second, to establish the degree of concordance between ACT and PSG recordings undertaken simultaneously in the same individuals. Here we examined sleep in the blue wildebeest, in a naturalistic setting, using both polysomnography (PSG) and actigraphy (ACT). PSG showed that total sleep time (TST) in the blue wildebeest for a 24-h period was 4.53 h (±0.12 h), 4.26 h (±0.11 h) spent in slow wave (non-REM) sleep and 0.28 h (±0.01 h) spent in rapid eye movement (REM) sleep, with 19.47 h (±0.12 h) spent in Wake. ACT showed that the blue wildebeest spent 19.23 h (±0.18 h) Active and 4.77 h (±0.18 h) Inactive. For both animals studied, a fair agreement between the two techniques for sleep scoring was observed, with approximately 45% of corresponding epochs analyzed being scored as both sleep (using PSG) and inactive (using ACT).
大多数关于哺乳动物睡眠的研究是在实验室/动物园设施的可控条件下进行的,很少有研究在其自然环境中开展。在动物的自然环境中通过多导睡眠图(PSG)记录睡眠情况并不总是可行的,因为PSG具有侵入性,需要在动物大脑表面进行电极的手术植入。相比之下,活动记录仪(ACT)已被证明是一种微创方法,可客观测量一些哺乳动物的总体睡眠时间,尽管无法揭示具体的睡眠状态。本研究的目的有两个,其一,在尽可能自然的条件下,通过多导睡眠图测量自由放养的蓝角马的睡眠情况;其二,确定在同一动物个体上同时进行的ACT和PSG记录之间的一致程度。在这里,我们在自然环境中,使用多导睡眠图(PSG)和活动记录仪(ACT)对蓝角马的睡眠进行了研究。PSG显示,蓝角马在24小时内的总睡眠时间(TST)为4.53小时(±0.12小时),其中慢波(非快速眼动)睡眠时长为4.26小时(±0.11小时),快速眼动(REM)睡眠时长为0.28小时(±0.01小时),清醒时长为19.47小时(±0.12小时)。ACT显示,蓝角马的活跃时长为19.23小时(±0.18小时),不活跃时长为4.77小时(±0.18小时)。对于所研究的两只动物,两种睡眠评分技术之间观察到了较好的一致性,约45%的相应分析时段在PSG中被评为睡眠,在ACT中被评为不活跃。