Key Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China.
State Key Lab High Efficiency Utilizat Coal & Gre, Natl Demonstrat Ctr Expt Chem Educ, Coll Chem & Chem Engn, Ningxia University, Yinchuan 750021, China.
Biosensors (Basel). 2021 Jun 24;11(7):207. doi: 10.3390/bios11070207.
Owing to the lack of specific diagnostic methods, Scrub typhus can sometimes be difficult to diagnose in the Asia-Pacific region. Therefore, an efficient and rapid detection method urgently needs to be developed. Based on competitive single-stranded DNA over modified glassy carbon electrode (GCE), an electrochemical biosensor was established to detect the disease. The nano-flower NiFe layered double hydroxide (NiFe-LDH) modified GCE has a large specific surface area, which supported a large amount of gold nanoparticles, so that a wide linear detection range from 25 fM to 0.5 μM was obtained. The beacon DNA (B-DNA) with the same sequence as the Scrub typhus DNA (T-DNA), but labeled with methylene blue, was used to construct a competitive relationship. When T-DNA and B-DNA were present on the sensor simultaneously, they would hybridize with probe DNA in a strong competition, and the corresponding electrochemical response signal would be generated via testing. It contributed to reducing tedious experimental procedures and excessive response time, and achieved fast electrochemical detection of DNA. The strategy provides a worthy avenue and possesses promising applications in disease diagnosis.
由于缺乏特定的诊断方法，恙虫病在亚太地区有时难以诊断。因此，迫切需要开发一种高效、快速的检测方法。基于修饰玻碳电极（GCE）上的竞争性单链 DNA，建立了一种电化学生物传感器来检测该疾病。纳米花状 NiFe 层状双氢氧化物（NiFe-LDH）修饰的 GCE 具有较大的比表面积，支持大量的金纳米粒子，从而获得了从 25 fM 到 0.5 μM 的宽线性检测范围。与恙虫病 DNA（T-DNA）具有相同序列但标记有亚甲基蓝的信标 DNA（B-DNA）被用来构建竞争关系。当传感器上同时存在 T-DNA 和 B-DNA 时，它们会与探针 DNA 发生强烈的杂交竞争，通过检测会产生相应的电化学响应信号。这有助于减少繁琐的实验程序和过长的响应时间，实现了 DNA 的快速电化学检测。该策略为疾病诊断提供了一个有价值的途径，并具有广阔的应用前景。
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