Department of Analytical Chemistry, Wroclaw Medical University, Borowska 211, PL-50556 Wrocław, Poland.
Department of Toxicology, Wroclaw Medical University, Borowska 211, PL-50556 Wrocław, Poland.
Molecules. 2021 Jun 24;26(13):3869. doi: 10.3390/molecules26133869.
In this research, the selected drugs commonly used in diabetes and its comorbidities (gliclazide, cilazapril, atorvastatin, and acetylsalicylic acid) were studied for their interactions with bovine serum albumin-native and glycated. Two different spectroscopic methods, fluorescence quenching and circular dichroism, were utilized to elucidate the binding interactions of the investigational drugs. The glycation process was induced in BSA by glucose and was confirmed by the presence of advanced glycosylation end products (AGEs). The interaction between albumin and gliclazide, with the presence of another drug, was confirmed by calculation of association constants (0.11-1.07 × 10 M). The nature of changes in the secondary structure of a protein depends on the drug used and the degree of glycation. Therefore, these interactions may have an influence on pharmacokinetic parameters.
在这项研究中，研究了常用于糖尿病及其合并症的药物（格列齐特、西拉普利、阿托伐他汀和乙酰水杨酸）与牛血清白蛋白天然和糖化的相互作用。利用两种不同的光谱方法，荧光猝灭和圆二色性，阐明了研究药物的结合相互作用。通过葡萄糖诱导 BSA 的糖化过程，并通过存在晚期糖基化终产物 (AGE) 来证实。通过计算结合常数（0.11-1.07×10 M），确认了白蛋白与格列齐特的相互作用，以及另一种药物的存在。蛋白质二级结构变化的性质取决于所用药物和糖化程度。因此，这些相互作用可能会影响药代动力学参数。
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