Beghin Mahaut, Schmitz Mélodie, Betoulle Stéphane, Palluel Olivier, Baekelandt Sébastien, Mandiki Syaghalirwa N M, Gillet Erin, Nott Katherine, Porcher Jean-Marc, Robert Christelle, Ronkart Sébastien, Kestemont Patrick
Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth and Environment, University of Namur, 61 Rue de Bruxelles, B-5000 Namur, Belgium.
Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth and Environment, University of Namur, 61 Rue de Bruxelles, B-5000 Namur, Belgium.
Ecotoxicol Environ Saf. 2021 Sep 15;221:112454. doi: 10.1016/j.ecoenv.2021.112454. Epub 2021 Jun 29.
Pharmaceuticals are emerging pollutants of concern for aquatic ecosystems where they are occurring in complex mixtures. In the present study, the chronic toxicity of an environmentally relevant pharmaceutical mixture on juvenile rainbow trout (Oncorhynchus mykiss) was investigated. Five pharmaceuticals (paracetamol, carbamazepine, diclofenac, naproxen and irbesartan) were selected based on their detection frequency and concentration levels in the Meuse river (Belgium). Fish were exposed for 42 days to three different concentrations of the mixture, the median one detected in the Meuse river, 10-times and 100-times this concentration. Effects on the nervous, immune, antioxidant, and detoxification systems were evaluated throughout the exposure period and their response standardized using the Integrated Biomarker Response (IBRv2) index. IBRv2 scores increased over time in the fish exposed to the highest concentration. After 42 days, fish exposed to the highest concentration displayed significantly higher levels in lysozyme activity (p < 0.01). The mixture also caused significant changes in brain serotonin turnover (p < 0.05). In short, our results indicate that the subchronic waterborne exposure to a pharmaceutical mixture commonly occurring in freshwater ecosystems may affect the neuroendocrine and immune systems of juvenile rainbow trout.
药物正成为水生生态系统中令人担忧的新兴污染物，它们以复杂混合物的形式存在。在本研究中，研究了一种与环境相关的药物混合物对幼年虹鳟（Oncorhynchus mykiss）的慢性毒性。根据它们在默兹河（比利时）中的检测频率和浓度水平，选择了五种药物（对乙酰氨基酚、卡马西平、双氯芬酸、萘普生和厄贝沙坦）。将鱼暴露于三种不同浓度的混合物中42天，分别是在默兹河中检测到的中位数浓度、该浓度的10倍和100倍。在整个暴露期间评估对神经、免疫、抗氧化和解毒系统的影响，并使用综合生物标志物反应（IBRv2）指数对它们的反应进行标准化。暴露于最高浓度的鱼的IBRv2得分随时间增加。42天后，暴露于最高浓度的鱼的溶菌酶活性水平显著更高（p < 0.01）。该混合物还导致大脑血清素周转率发生显著变化（p < 0.05）。简而言之，我们的结果表明，亚慢性水传播暴露于淡水生态系统中常见的药物混合物可能会影响幼年虹鳟的神经内分泌和免疫系统。
