Research Institute for Sustainable Humanosphere, Kyoto University.
Tohoku Medical Megabank Organization, Tohoku University.
Microbes Environ. 2021;36(3). doi: 10.1264/jsme2.ME21004.
Nitrogen deficiency affects soybean growth and physiology, such as symbiosis with rhizobia; however, its effects on the bacterial composition of the soybean root microbiota remain unclear. A bacterial community analysis by 16S rRNA gene amplicon sequencing showed nitrogen deficiency-induced bacterial community shifts in soybean roots with the marked enrichment of Methylobacteriaceae. The abundance of Methylobacteriaceae was low in the roots of field-grown soybean without symptoms of nitrogen deficiency. Although Methylobacteriaceae isolated from soybean roots under nitrogen deficiency did not promote growth or nodulation when inoculated into soybean roots, these results indicate that the enrichment of Methylobacteriaceae in soybean roots is triggered by nitrogen-deficiency stress.
氮缺乏会影响大豆的生长和生理特性，如与根瘤菌的共生；然而，其对大豆根微生物区系细菌组成的影响尚不清楚。通过 16S rRNA 基因扩增子测序进行的细菌群落分析表明，氮缺乏会引起大豆根细菌群落的变化，甲基杆菌科的丰度明显增加。在没有氮缺乏症状的田间生长的大豆根中，甲基杆菌科的丰度较低。尽管从氮缺乏的大豆根中分离出的甲基杆菌科在接种到大豆根中时不会促进生长或结瘤，但这些结果表明，大豆根中甲基杆菌科的富集是由氮胁迫引发的。
