Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
Department of Biochemistry, Microbiology and Immunology, Wayne State University School of Medicine, Detroit, Michigan, United States.
Invest Ophthalmol Vis Sci. 2021 Jul 1;62(9):12. doi: 10.1167/iovs.62.9.12.
To determine the role of scavenger receptor expressed by endothelial cell-1 (SREC-Ⅰ) in vitro and in a mouse model of Aspergillus fumigatus keratitis.
SREC-Ⅰ mRNA and protein expression were tested in both normal and A fumigatus stimulated human corneal epithelial cells (HCECs). Immunofluorescence was used to detect SREC-Ⅰ expression in human corneas with or without A fumigatus infection. HCECs were incubated with SREC-Ⅰ small interfering RNA, then the mRNA levels of LOX-1, IL-1β, and TNF-α were detected after A fumigatus stimulation. A mouse fungal keratitis (FK) model was established and SREC-Ⅰ mRNA and protein expression were detected by RT-PCR, Western blot and immunofluorescence. The severity of FK was evaluated by clinical score. CLCX1, LOX-1, IL-1β, and TNF-α mRNA expression levels were tested before and after anti-SREC-Ⅰ treatment.
SREC-Ⅰ expressed in normal and A fumigatus treated HCECs and human corneal epithelium. In vitro experiment showed that SREC-Ⅰ mRNA and protein levels were significantly increased after A fumigatus stimulation. SREC-Ⅰ small interfering RNA treatment inhibited the expressions of LOX-1, IL-1β, and TNF-α in HCECs. The expressions of CLCX1, LOX-1, IL-1β, and TNF-α were elevated in mice with A fumigatus keratitis, which could be decreased by SREC-Ⅰ-neutralizing antibody treatment.
SREC-Ⅰ is a key mediator in inflammatory response induced by A fumigatus keratitis. SREC-Ⅰ blockade could be a potential therapeutic approach for FK.
确定内皮细胞表达的清道夫受体Ⅰ（SREC-Ⅰ）在体外和烟曲霉角膜炎小鼠模型中的作用。
检测正常和烟曲霉刺激的人角膜上皮细胞（HCECs）中 SREC-Ⅰ mRNA 和蛋白的表达。免疫荧光法检测有无烟曲霉感染的人角膜中 SREC-Ⅰ的表达。用 SREC-Ⅰ小干扰 RNA 孵育 HCECs，然后在烟曲霉刺激后检测 LOX-1、IL-1β 和 TNF-α 的 mRNA 水平。建立小鼠真菌性角膜炎（FK）模型，通过 RT-PCR、Western blot 和免疫荧光检测 SREC-Ⅰ mRNA 和蛋白的表达。通过临床评分评估 FK 的严重程度。在抗 SREC-Ⅰ治疗前后检测 CLCX1、LOX-1、IL-1β 和 TNF-α 的 mRNA 表达水平。
SREC-Ⅰ在正常和烟曲霉处理的 HCECs 及人角膜上皮细胞中表达。体外实验表明，烟曲霉刺激后 SREC-Ⅰ mRNA 和蛋白水平明显升高。SREC-Ⅰ小干扰 RNA 处理抑制 HCECs 中 LOX-1、IL-1β 和 TNF-α 的表达。烟曲霉角膜炎小鼠中 CLCX1、LOX-1、IL-1β 和 TNF-α 的表达升高，SREC-Ⅰ中和抗体治疗可降低其表达。
SREC-Ⅰ是烟曲霉角膜炎诱导的炎症反应的关键介质。SREC-Ⅰ阻断可能是 FK 的一种潜在治疗方法。
