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微小RNA和信使核糖核酸转录组的综合分析揭示了对……和……反应的分子机制。 (注:原文中“and.”表述不完整,翻译时根据语境补充了“对……和……反应”)

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Response to and .

作者信息

Yue Hao, Huang Li-Ping, Lu Ding-Yi-Hui, Zhang Zhan-Hong, Zhang Zhuo, Zhang De-Yong, Zheng Li-Min, Gao Yang, Tan Xin-Qiu, Zhou Xu-Guo, Shi Xiao-Bin, Liu Yong

机构信息

Subcollege of Longping, Graduate School of Hunan University, Changsha, China.

Hunan Academy of Agricultural Sciences, Institute of Plant Protection, Changsha, China.

出版信息

Front Microbiol. 2021 Jun 22;12:693574. doi: 10.3389/fmicb.2021.693574. eCollection 2021.

Abstract

(ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus and is very limited. To understand the molecular mechanism of the interaction between tomato, ToCV and , we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection of and ToCV in tomato plants. Our data revealed that 6199 mRNAs were significantly regulated, and the differentially expressed genes were most significantly associated with the plant-pathogen interaction, the MAPK signaling pathway, the glyoxylate, and the carbon fixation in photosynthetic organisms and photosynthesis related proteins. Concomitantly, 242 differentially expressed miRNAs were detected, including novel putative miRNAs. Sly-miR159, sly-miR9471b-3p, and sly-miR162 were the most expressed miRNAs in each sample compare to control group. Moreover, we compared the similarities and differences of gene expression in tomato plant caused by infection or co-infection of and ToCV. Taken together, the analysis reported in this article lays a solid foundation for further research on the interaction between tomato, ToCV and , and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants.

摘要

番茄褪绿病毒(ToCV)是对栽培番茄危害最大的病毒之一,严重威胁着全球农作物的生长。作为ToCV的传播媒介,烟粉虱地中海生物型(MED)是ToCV迅速传播的主要原因。目前对番茄植株对这种病毒的反应的了解非常有限。为了了解番茄、ToCV和烟粉虱之间相互作用的分子机制,我们采用了下一代测序方法来破译在番茄植株受烟粉虱和ToCV感染时差异表达的miRNA和mRNA。我们的数据显示,6199个mRNA受到显著调控,差异表达基因与植物-病原体相互作用、丝裂原活化蛋白激酶(MAPK)信号通路、乙醛酸和光合生物中的碳固定以及光合作用相关蛋白最为显著相关。同时,检测到242个差异表达的miRNA,包括新的假定miRNA。与对照组相比,sly-miR159、sly-miR9471b-3p和sly-miR162是每个样本中表达量最高的miRNA。此外,我们比较了由烟粉虱感染或烟粉虱与ToCV共同感染引起的番茄植株基因表达的异同。综上所述,本文报道的分析为进一步研究番茄、ToCV和烟粉虱之间的相互作用奠定了坚实基础,并为鉴定影响番茄植株中病毒传播的潜在关键基因提供了证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c44/8258350/03ad06454466/fmicb-12-693574-g001.jpg

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