超高场磁共振成像测定不同年龄人晶状体中的水扩散速率。

Ultrahigh field MRI determination of water diffusion rates in human lenses of different age.

作者信息

Stahnke Thomas, Lindner Tobias, Guthoff Rudolf, Stachs Oliver, Wree Andreas, Langner Sönke, Niendorf Thoralf, Grabow Niels, Glass Änne, Beller Ebba, Polei Stefan

机构信息

Department of Ophthalmology, Rostock University Medical Center, Rostock, Germany.

Core Facility Multimodal Small Animal Imaging, Rostock University Medical Center, Rostock, Germany.

出版信息

Quant Imaging Med Surg. 2021 Jul;11(7):3029-3041. doi: 10.21037/qims-20-1124.

DOI:10.21037/qims-20-1124

PMID:34249632

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8250020/

Abstract

BACKGROUND

The development of presbyopia is correlated with increased lens stiffness. To reveal structural changes with age, ultrahigh field magnetic resonance imaging (UHF-MRI) was used to analyze water diffusion in differently aged human lenses .

METHODS

After enucleation lens extractions were performed. Lenses were photographed, weighed, and embedded in 0.5% agarose dissolved in culture medium. UHF-MRI was conducted to analyze anatomical characteristics of the lens using T2-weighted Turbo-RARE imaging and to obtain apparent diffusion coefficients (ADC) measurements. A Gaussian fit routine was used to examine the ADC histograms.

RESULTS

An age-dependent increase in lens wet weight, lens thickness, and lens diameter was found (P<0.001). T2-weighted images revealed a hyperintense lens cortex and a gradually negative gradient in signal intensity towards the nucleus. ADC histograms of the lens showed bimodal distributions (lower ADC values mainly located in the nucleus and higher ADC values mainly located in the cortex), which did not change significantly with age [βPeak1=1.96E-7 (-20E-7, 10E-7), P=0.804 or βPeak2=15.4E-7 (-10E-7, 40E-7), P=0.276; respectively].

CONCLUSIONS

Clinically relevant age dependent lens hardening is probably not correlated with ADC changes within the nucleus, which could be confirmed by further measurements.

摘要

背景

老花眼的发展与晶状体硬度增加相关。为了揭示随年龄增长的结构变化，采用超高场磁共振成像（UHF-MRI）分析不同年龄人晶状体中的水扩散情况。

方法

眼球摘除后进行晶状体摘除。对晶状体进行拍照、称重，并嵌入溶解于培养基中的0.5%琼脂糖中。使用UHF-MRI通过T2加权快速自旋回波成像分析晶状体的解剖特征，并获得表观扩散系数（ADC）测量值。使用高斯拟合程序检查ADC直方图。

结果

发现晶状体湿重、晶状体厚度和晶状体直径随年龄增长而增加（P<0.001）。T2加权图像显示晶状体皮质呈高信号，向核部信号强度逐渐呈负梯度。晶状体的ADC直方图显示双峰分布（较低的ADC值主要位于核部，较高的ADC值主要位于皮质），且不随年龄显著变化[β峰1=1.96E-7（-20E-7，10E-7），P=0.804；或β峰2=15.4E-7（-10E-7，40E-7），P=0.276]。