Masuzawa M, Hamazaki H, Nishioka K, Nishiyama S, Ryan T J
Department of Dermatology, Kitasato University School of Medicine, Kanagawa, Japan.
Biochem Biophys Res Commun. 1987 Dec 31;149(3):866-73. doi: 10.1016/0006-291x(87)90488-8.
UK-I, a 60-kDa urokinase-inhibitor derived from human fibroblasts, inhibited 54-kDa urokinase (EC 3.4.21.31) activity dose-dependently on ordinary fibrin-agar autograms. This UK-I formed an SDS-stable approximately 75-kDa complex with radioiodinated urokinase (33 kDa) on an autoradiogram following SDS/polyacrylamide gel electrophoresis. Benzamidine hydrochloride inhibited its formation, indicating UK-I to bind at the active site of urokinase and form an inactive complex. UK-I did not form a complex with [125I]thrombin (EC 3.4.21.5). It is thus evident that UK-I is one type of urokinase-inhibitor derived from human fibroblasts with properties differing from protease nexin, another urokinase-inhibitor derived from the same source.
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