环状 RNA PRKCI/miR-24-3p 在前列腺癌转移中的作用。
The role of Circ_PRKCI/miR-24-3p in the metastasis of prostate cancer.
机构信息
Department of Urology, The First Affiliated Hospital of Jiangxi Medical College, Shangrao 334000, China.
出版信息
J BUON. 2021 May-Jun;26(3):949-955.
PURPOSE
The purpose of this study was to explore the role of circ_PRKCI in regulating prostate cancer (PCa) development and the underlying molecular mechanism.
METHODS
Circ_PRKCI levels in 40 PCa tissues and adjacent normal ones were detected. The relationship between circ_PRKCI level and pathological indicators in PCa patients was explored. After transfection of sh-circ_PRKCI in DU-145 and PC-3 cells, changes in viability, numbers of migratory and invasive cells, and wound closure were examined. Finally, the downstream target of circ_PRKCI was confirmed by dual-luciferase reporter assay and their involvement in PCa development was finally illustrated by rescue experiments.
RESULTS
Circ_PRKCI was upregulated in PCa tissues than adjacent normal ones. PCa patients expressing a high level of circ_PRKCI had high risks of lymphatic metastasis and distant metastasis. Knockdown of circ_PRKCI weakened proliferative and metastatic abilities of PCa cells. As the downstream target of circ_PRKCI, miR-24-3p was negatively regulated by it. Moreover, circ_PRKCI/miR-24-3p axis was responsible for triggering proliferative and metastatic potentials in PCa.
CONCLUSIONS
Circ_PRKCI is upregulated in PCa tissues, and its level is linked to metastasis rate in PCa patients. It triggers proliferative and metastatic potentials in PCa by downregulating miR-24-3p.
目的
本研究旨在探讨环状 PRKCI 在调节前列腺癌（PCa）发展中的作用及其潜在的分子机制。
方法
检测 40 例 PCa 组织和相邻正常组织中的环状 PRKCI 水平。探讨环状 PRKCI 水平与 PCa 患者病理指标之间的关系。在 DU-145 和 PC-3 细胞中转染 sh-circ_PRKCI 后，检测细胞活力、迁移和侵袭细胞数量以及伤口愈合的变化。最后，通过双荧光素酶报告实验证实环状 PRKCI 的下游靶基因，并通过挽救实验阐明其在 PCa 发展中的作用。
结果
环状 PRKCI 在 PCa 组织中上调，高于相邻正常组织。表达高水平环状 PRKCI 的 PCa 患者具有较高的淋巴转移和远处转移风险。环状 PRKCI 的敲低削弱了 PCa 细胞的增殖和迁移能力。作为环状 PRKCI 的下游靶基因，miR-24-3p 受到其负调控。此外，环状 PRKCI/miR-24-3p 轴负责触发 PCa 的增殖和迁移潜能。
结论
环状 PRKCI 在 PCa 组织中上调，其水平与 PCa 患者的转移率相关。通过下调 miR-24-3p，环状 PRKCI 触发了 PCa 的增殖和迁移潜能。
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