Piera Lucyna, Szymański Jacek, Juszczak Marlena, Drobnik Jacek
Laboratory of Connective Tissue Metabolism, Department of Pathophysiology, Medical University of Łódź, 90-752 Łódź, Poland.
Central Scientific Laboratory, Medical University of Łódź, 92-215 Łódź, Poland.
Biomed Rep. 2021 Aug;15(2):71. doi: 10.3892/br.2021.1447. Epub 2021 Jul 5.
Histamine is involved in the regulation of collagen metabolism during healing following a myocardial infarction; however, its effects on the intact heart tissue is unknown. The aim of the present study was to determine whether histamine may influence collagen content in cells isolated from intact heart, and to identify the histamine receptor involved in the regulation of collagen deposition. Cells were isolated from intact rat hearts and subjected to identification by flow cytometry. The effects of histamine and its receptor agonists and antagonists were investigated. The heart cells were found to be actin, desmin and vimentin positive. Histamine (used at a concentrations of 1x10-1x10 M) increased collagen content within the culture and increased the expression of α1 chain of the procollagen type III gene. The H, H and H receptor inhibitors ranitidine, ciproxifan and JNJ 7777120 blocked the effect of histamine on collagen content. All tested histamine receptor agonists, 2-pyridylethylamine dihydrochloride (H receptor agonist), amthamine dihydrobromide (H receptor agonist), imetit (H receptor agonist) and 4-methylhistamine hydrochloride (H receptor agonist), elevated collagen content within the heart myofibroblast cultures. The cells isolated from the intact heart were identified as myofibroblasts. Thus, the results of the present study showed that histamine augmented collagen content in the heart myofibroblast culture by activation of three histamine receptors (H, H and H). The effect of the amine was also dependent on the activation of collagen type III gene expression.
组胺参与心肌梗死后愈合过程中胶原代谢的调节；然而，其对完整心脏组织的影响尚不清楚。本研究的目的是确定组胺是否可能影响从完整心脏分离的细胞中的胶原含量，并确定参与调节胶原沉积的组胺受体。从完整的大鼠心脏中分离细胞，并通过流式细胞术进行鉴定。研究了组胺及其受体激动剂和拮抗剂的作用。发现心脏细胞肌动蛋白、结蛋白和波形蛋白呈阳性。组胺（使用浓度为1×10⁻¹×10 M）增加了培养物中的胶原含量，并增加了Ⅲ型前胶原α1链基因的表达。H₁、H₂和H₃受体抑制剂雷尼替丁、西普昔芬和JNJ 7777120阻断了组胺对胶原含量的影响。所有测试的组胺受体激动剂，2-吡啶乙胺二盐酸盐（H₁受体激动剂）、氨他明二氢溴酸盐（H₂受体激动剂）、咪替丁（H₃受体激动剂）和4-甲基组胺盐酸盐（H₄受体激动剂），均提高了心脏肌成纤维细胞培养物中的胶原含量。从完整心脏分离的细胞被鉴定为肌成纤维细胞。因此，本研究结果表明，组胺通过激活三种组胺受体（H₁、H₂和H₃）增加了心脏肌成纤维细胞培养物中的胶原含量。胺的作用还依赖于Ⅲ型胶原基因表达的激活。
