State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Agricultural Genome Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Int J Mol Sci. 2021 Jul 2;22(13):7157. doi: 10.3390/ijms22137157.
To reveal the mechanism of temperature preference in , one of the top 20 plant pests in the world, we cloned and identified , , and genes by RACE and bioinformatic analysis, and clarified their expression profiles during different development stages using real-time PCR, and revealed their function in preference temperature by RNAi. The full-length cDNA of was 3136 bp, with a 2865-bp open reading frame encoding a 259.89-kDa protein; and the partial length cDNA of was 2326-bp, with a 2025-bp open reading frame encoding a 193.16-kDa protein. In addition, the expression of and was significantly lower in larvae than other stages, and it was significantly higher in pupae and newly emerging males for . After feeding target double-stranded RNA (dsRNA), the preferred temperature decreased 2 °C more than the control group. In conclusion, the results firstly indicated the molecular characterization of TRPA subfamily genes and their key role in temperature perception in , and the study will help us to understand the temperature-sensing mechanism in the pest, and will provide some basis for study of other Lepidoptera insects' temperature preference. Moreover, it is of great significance in enriching the research progress of "thermos TRP".
为了揭示世界 20 大植物害虫之一的温度偏好机制，我们通过 RACE 和生物信息学分析克隆并鉴定了 、 和 基因，并通过实时 PCR 阐明了它们在不同发育阶段的表达谱，通过 RNAi 揭示了它们在偏好温度中的功能。 的全长 cDNA 为 3136 bp，开放阅读框为 2865 bp，编码 259.89 kDa 的蛋白质;部分长度 cDNA 为 2326 bp，开放阅读框为 2025 bp，编码 193.16 kDa 的蛋白质。此外， 和 的表达在幼虫期明显低于其他阶段，而 在蛹期和新出现的雄性中明显较高。喂食靶双链 RNA(dsRNA)后，实验组比对照组更偏好的温度降低了 2°C。总之，该结果首次表明了 TRPA 亚家族基因在 中的分子特征及其在温度感知中的关键作用，该研究将有助于我们了解害虫的温度感应机制，并为研究其他鳞翅目昆虫的温度偏好提供一些依据。此外，这对丰富“热敏 TRP”的研究进展具有重要意义。
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