壳聚糖添加对熊果酸非离子囊泡细胞摄取和细胞毒性的影响。
The effect of chitosan addition on cellular uptake and cytotoxicity of ursolic acid niosomes.
机构信息
Universitas Airlangga, Faculty of Pharmacy, Department of Pharmaceutical Sciences, Nanizar Zaman Joenoes Building, Campus C Mulyorejo, Surabaya, 60115 Indonesia.
Universitas Airlangga, Stem Cell Research and Development Center, Institute of Tropical Disease Center Building, Campus C Mulyorejo, Surabaya, 60115 Indonesia.
出版信息
An Acad Bras Cienc. 2021 Jul 19;93(3):e20201850. doi: 10.1590/0001-3765202120201850. eCollection 2021.
This study evaluated the cellular uptake and cytotoxicity of low permeable Ursolic acid (UA) on cancer cells using niosomes composed of span 60 and cholesterol. The results showed that the addition of chitosan increased particle sizes and ζ-potentials. The UA niosomes with chitosan layers had higher cytotoxicity in HeLa cells than without chitosan, however, there was no improvement observed for Huh7it cells. Moreover, chitosan layers improved the cellular uptake, which clathrin-mediated endocytosis may determine the cellular transport of UA niosomes. In conclusion, the addition of chitosan improved cellular uptake and cytotoxicity of UA niosomes in the HeLa cells.
本研究评估了由 Span 60 和胆固醇组成的脂质体对低渗透性熊果酸（UA）在癌细胞中的细胞摄取和细胞毒性。结果表明，壳聚糖的添加增加了颗粒大小和 ζ-电位。与不含壳聚糖的脂质体相比，具有壳聚糖层的 UA 脂质体在 HeLa 细胞中的细胞毒性更高，然而，在 Huh7it 细胞中没有观察到改善。此外，壳聚糖层提高了细胞摄取，网格蛋白介导的内吞作用可能决定了 UA 脂质体的细胞转运。总之，壳聚糖的添加提高了 UA 脂质体在 HeLa 细胞中的细胞摄取和细胞毒性。
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