College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, People's Republic of China.
Mikrochim Acta. 2021 Jul 21;188(8):266. doi: 10.1007/s00604-021-04924-9.
An ultrasensitive electrochemical sensor has been constructed for the detection of single nucleotide polymorphisms (SNPs) based on DNA-functionalized Cd-MOFs-74 as cascade signal amplification probe under enzyme-free conditions. Interestingly, the introduction of an auxiliary probe did not disturb the detection of SNP targets, but could bind more Cd-MOFs-74 signal elements to enhance the different pulse voltammetry electrochemical signal 2~3 times as compared to sensing system without auxiliary probe, which obviously improves the sensitivity of the proposed sensor. Experimental results taking p53 tumor suppressor gene as SNP model demonstrated that the proposed method can be employed to sensitively and selectively detect target p53 gene fragment with a linear response ranging from 0.01 to 30 pmol/L (detection limit of 6.3 fmol/L) under enzyme-free conditions. Utilizing this strategy, the ultrasensitive SNP electrochemical sensor is a promising tool for the determination of SNPs in biomedicine. Graphical Abstract.
一种基于 DNA 功能化 Cd-MOFs-74 的超灵敏电化学传感器已构建用于在无酶条件下对单核苷酸多态性 (SNP) 的检测。有趣的是，辅助探针的引入并没有干扰 SNP 靶标的检测，反而可以结合更多的 Cd-MOFs-74 信号元件，与没有辅助探针的传感系统相比，增强了不同脉冲伏安电化学信号 2~3 倍，明显提高了传感器的灵敏度。以 p53 肿瘤抑制基因作为 SNP 模型的实验结果表明，该方法可用于在无酶条件下灵敏、选择性地检测靶标 p53 基因片段，线性响应范围为 0.01 至 30 pmol/L（检测限为 6.3 fmol/L）。利用该策略，超灵敏 SNP 电化学传感器有望成为生物医学中 SNP 测定的有力工具。
