乙型肝炎表面抗原固相反向被动血凝吸附试验
Solid phase reverse passive hemadsorption test for hepatitis B surface antigen.
作者信息
Duverlie G, Driencourt M, Roussel C, Daniel P
机构信息
Laboratoire de Virologie, Centre Hospitalo-Universitaire, Amiens, France.
出版信息
J Virol Methods. 1987 Nov;18(2-3):107-12. doi: 10.1016/0166-0934(87)90115-7.
A solid phase reverse passive hemadsorption test (SP-RPHAd) for hepatitis B surface antigen detection is described. It was compared with a commercial reverse passive hemagglutination assay (Hepatest, Wellcome, U.K.). SP-RPHAd is four-fold less expensive than Hepatest and undiluted sera can be used instead of eight-fold diluted sera without risk of non-specific hemagglutination. Thus, the threshold of detection is lowered to about 5 ng/ml by SP-RPHAd.
本文描述了一种用于检测乙型肝炎表面抗原的固相反向被动血凝吸附试验（SP-RPHAd）。将其与一种商业反向被动血凝试验（Hepatest，英国威康公司）进行了比较。SP-RPHAd的成本比Hepatest低四倍，且无需使用八倍稀释的血清，未稀释的血清即可使用，不会有非特异性血凝的风险。因此，SP-RPHAd将检测阈值降低至约5 ng/ml。
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