利用 miRNA 开关从人多能干细胞中分离心肌细胞。

Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells Using miRNA Switches.

机构信息

Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.

Department of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.

出版信息

Methods Mol Biol. 2021;2320:35-51. doi: 10.1007/978-1-0716-1484-6_5.

DOI:10.1007/978-1-0716-1484-6_5

PMID:34302646

Abstract

The most common method for isolating cells of interest is an antibody method that recognizes cell surface antigens. However, specific surface antigens for many cell types have not been identified. We have developed the microRNA (miRNA)-responsive synthetic mRNA systems (miRNA switches), which isolate target cells based on endogenous miRNA activity. In this chapter, we describe protocols for isolating human pluripotent stem cell (hPSC)-derived cardiomyocytes using miRNA switches with or without cell sorting.

摘要

最常用的分离目的细胞的方法是抗体法，该方法识别细胞表面抗原。然而，许多细胞类型的特异性表面抗原尚未被鉴定。我们已经开发出基于内源性 miRNA 活性分离靶细胞的 microRNA（miRNA）响应合成 mRNA 系统（miRNA 开关）。在本章中，我们描述了使用 miRNA 开关（有或没有细胞分选）分离人多能干细胞（hPSC）衍生的心肌细胞的方案。