人源微小RNA-330-5p通过靶向叉头框E1加重甲状腺癌。

Hsa-miR-330-5p Aggravates Thyroid Carcinoma via Targeting FOXE1.

作者信息

Wang Yuxia, Liu Zhaozhe, Ren Xiangning, Sun Nan, Li Qiuhua, Bian Che

机构信息

Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, Liaoning, China.

Oncology Department, General Hospital of Northern Theater Command, Shenyang 110014, Liaoning, China.

出版信息

J Oncol. 2021 Jul 2;2021:1070365. doi: 10.1155/2021/1070365. eCollection 2021.

DOI:10.1155/2021/1070365

PMID:34306074

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8272668/

Abstract

BACKGROUND

Thyroid carcinoma (TC) is one of the frequent endocrine malignancies, and growing evidence suggests that aberrant microRNA (miRNA) expression contributes to TC development and progression. Nevertheless, the function of miR-330-5p in the progression of TC remains unknown.

METHODS

The expression levels of miR-330-5 in patients with thyroid carcinoma and healthy controls were detected, and their potential diagnostic and prognostic values were analyzed.

RESULTS

In this study, we firstly found that miR-330-5p expression was markedly upregulated in TC tissue and cell lines. Functionally, the downregulation of miR-330-5p suppressed TC cell proliferation, migration, and invasion. Further studies revealed that miR-330-5p negatively regulated the expression of forkhead box E1 (FOXE1). More importantly, the results of rescue experiments suggested that FOXE1 overexpression reduced the positive effects of miR-330-5p overexpression in TPC-1 and K-1 cells.

CONCLUSIONS

This work revealed that miR-330-5p facilitated the TC progression through targeting FOXE1, which may offer novel therapeutic options for TC.

摘要

背景

甲状腺癌（TC）是常见的内分泌恶性肿瘤之一，越来越多的证据表明，异常的微小RNA（miRNA）表达促进了TC的发生和发展。然而，miR-330-5p在TC进展中的作用仍不清楚。

方法

检测甲状腺癌患者和健康对照者中miR-330-5的表达水平，并分析其潜在的诊断和预后价值。

结果

在本研究中，我们首先发现miR-330-5p在TC组织和细胞系中显著上调。在功能上，miR-330-5p的下调抑制了TC细胞的增殖、迁移和侵袭。进一步研究表明，miR-330-5p负向调节叉头框E1（FOXE1）的表达。更重要的是，挽救实验结果表明，FOXE1过表达降低了miR-330-5p过表达对TPC-1和K-1细胞的正向作用。