Kinner Alina, Rosenthal Katrin, Lütz Stephan
Chair for Bioprocess Engineering, Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany.
Front Bioeng Biotechnol. 2021 Jul 7;9:705630. doi: 10.3389/fbioe.2021.705630. eCollection 2021.
In 2004, the fungal heme-thiolate enzyme subfamily of unspecific peroxygenases (UPOs) was first described in the basidiomycete . As UPOs naturally catalyze a broad range of oxidative transformations by using hydrogen peroxide as electron acceptor and thus possess a great application potential, they have been extensively studied in recent years. However, despite their versatility to catalyze challenging selective oxyfunctionalizations, the availability of UPOs for potential biotechnological applications is restricted. Particularly limiting are the identification of novel natural biocatalysts, their production, and the description of their properties. It is hence of great interest to further characterize the enzyme subfamily as well as to identify promising new candidates. Therefore, this review provides an overview of the state of the art in identification, expression, and screening approaches of fungal UPOs, challenges associated with current protein production and screening strategies, as well as potential solutions and opportunities.
2004年，非特异性过氧酶（UPOs）的真菌血红素硫醇盐酶亚家族首次在担子菌中被描述。由于UPOs通过使用过氧化氢作为电子受体自然催化广泛的氧化转化反应，因此具有巨大的应用潜力，近年来受到了广泛研究。然而，尽管它们具有催化具有挑战性的选择性氧官能化反应的多功能性，但UPOs在潜在生物技术应用中的可用性受到限制。特别受限的是新型天然生物催化剂的鉴定、生产及其性质的描述。因此，进一步表征该酶亚家族以及鉴定有前景的新候选物具有极大的意义。因此，本综述概述了真菌UPOs的鉴定、表达和筛选方法的现状、当前蛋白质生产和筛选策略相关的挑战以及潜在的解决方案和机会。
