Medical University of Vienna, Vienna, Austria.
Acta Ophthalmol. 2022 Mar;100(2):e409-e413. doi: 10.1111/aos.14981. Epub 2021 Jul 26.
To evaluate the effects of femtosecond laser-assisted keratoplasty using a liquid patient interface (L-PI) as opposed to an applanated interface (A-PI) on graft quality and functionality markers.
Pressure measurements during femtosecond laser-assisted trephination were performed using two groups of 10 porcine eyes. Trephination was performed either in an L-PI or in an A-PI setting. Pressure sensor needles placed intravitreally continuously recorded intraocular pressure during trephination. Twenty paired human donor eyes were used to test the morphological quality of donor tissue after trephination in L-PI and A-PI settings. Optical coherence tomography (OCT) scans were performed before and after trephination. Images were processed using ImageJ and pixel .
During trephination, pressure measurements with an L-PI were significantly lower than with an A-PI (p = 0.0121). Mean pressure during trephination was 78.1 mmHg ± 37.6 mmHg with L-PI and 188.6 mmHg ± 17.7 mmHg with A-PI. Trephination in A-PI produced a significantly larger increase (p < 0.00001) in donor pachymetry than trephination in L-PI. Significantly lower areas of Descemet folds were achieved in L-PI trephination than in A-PI trephination (p < 0.01). There was no significant difference in circularity between A-PI and L-PI (p = 0.27). Total time required for trephination was comparable between L-PI and A-PI (p = 0.45). Time taken to reach working vacuum was achieved significantly more quickly in L-PI (p < 0.05).
Femtosecond laser-assisted L-PI keratoplasty appears to be a promising method to decrease stress to donor and recipient tissue during femtosecond laser-assisted trephination. Results showed favourable donor tissue morphology markers after L-PI trephination.
评估飞秒激光辅助角膜移植术使用液体患者界面（L-PI）与压平界面（A-PI）在移植物质量和功能标记物方面的效果。
使用两组 10 只猪眼进行飞秒激光辅助环钻术时的压力测量。在 L-PI 或 A-PI 设置下进行环钻术。放置在眼内的压力传感器针在环钻术期间连续记录眼内压。使用 20 对人供体眼角膜测试 L-PI 和 A-PI 设置中环钻术后供体组织的形态质量。在环钻术前和术后进行光学相干断层扫描（OCT）扫描。使用 ImageJ 软件对图像进行处理和像素分析。
在环钻术中，L-PI 的压力测量值明显低于 A-PI（p=0.0121）。L-PI 中环钻术中的平均压力为 78.1mmHg±37.6mmHg，而 A-PI 中环钻术中的平均压力为 188.6mmHg±17.7mmHg。A-PI 中环钻术导致供体角膜厚度的增加明显大于 L-PI 中环钻术（p<0.00001）。L-PI 中环钻术获得的 Descemet 褶皱面积明显小于 A-PI 中环钻术（p<0.01）。A-PI 和 L-PI 之间的圆度没有显著差异（p=0.27）。L-PI 和 A-PI 中环钻术所需的总时间相当（p=0.45）。在 L-PI 中环钻术达到工作真空所需的时间明显更快（p<0.05）。
飞秒激光辅助 L-PI 角膜移植术似乎是一种有前途的方法，可以在飞秒激光辅助环钻术中减少供体和受体组织的压力。结果表明，L-PI 环钻术后供体组织形态学标志物良好。
