三抗生素药物（3Mix-MP）联合聚乙二醇、传统三联抗生素糊剂、氢氧化钙及蜂胶乙醇提取物的抗菌效果：离体管内牙本质共聚焦激光扫描显微镜研究。

Antibacterial Efficacy of Triple Antibiotic Medication With Macrogol (3Mix-MP), Traditional Triple Antibiotic Paste, Calcium Hydroxide, and Ethanol Extract of Propolis: An Intratubular Dentin Ex Vivo Confocal Laser Scanning Microscopic Study.

机构信息

PROCLIN Department, School of Dentistry, Rio de Janeiro State University, Rio de Janeiro, Rio de Janeiro, Brazil.

Dentistry, Endodontics, and Dental Materials Department, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.

出版信息

J Endod. 2021 Oct;47(10):1609-1616. doi: 10.1016/j.joen.2021.07.014. Epub 2021 Jul 24.

DOI:10.1016/j.joen.2021.07.014

PMID:34310981

Abstract

INTRODUCTION

This study aimed to assess the effectiveness of antibacterial activity of medications used in regenerative endodontic treatment.

METHODS

Sixty-seven dentin cylinders of single-rooted teeth were contaminated with a culture of Enterococcus faecalis (ATCC 29212; American Type Culture Collection, Manassas, VA) for 5 days. Samples were divided into 1 control group and the following experimental groups according to the medication applied: traditional triple antibiotic paste (TAP), clindamycin-modified TAP (mTAP), triple antibiotic medication with macrogol (3Mix-MP), clindamycin-modified 3Mix-MP (m3Mix-MP), calcium hydroxide (CH), and ethanol extract of propolis (EEP). After 14 days, the medications were removed, and the samples were submitted to confocal laser scanning microscopic analysis to quantify the percentage of viable bacteria. The distribution of data was confirmed by the Shapiro-Wilk test. The Kruskal-Wallis and Dunn tests were used for intergroup comparisons, and the Wilcoxon test was used for comparison between superficial and deep antibacterial efficacy for the same medication. The level of significance was set at P < .05.

RESULTS

3Mix-MP and m3Mix-MP presented significantly higher antibacterial efficacy compared with the other tested medications (P < .05), except for mTAP. mTAP was more effective than TAP (P < .05). The antibacterial efficacy of EEP and CH did not differ significantly from TAP and mTAP (P > .05). All medications showed effective antibacterial action compared with the control group (P < .05).

CONCLUSIONS

3Mix-MP and m3Mix-MP, which present extremely high concentrations of antibiotics (1500 mg/mL), were not more effective than mTAP at the concentration recommended by the American Association of Endodontists (5 mg/mL). Moreover, CH and EEP were as effective as TAP and mTAP.

摘要

引言

本研究旨在评估再生牙髓治疗中使用的药物的抗菌活性。

方法

将 67 个单根牙的牙本质圆柱用粪肠球菌（ATCC 29212；美国典型培养物保藏中心，弗吉尼亚州马纳萨斯）的培养物污染 5 天。根据所使用的药物将样本分为 1 个对照组和以下实验组：传统三联抗生素糊剂（TAP）、克林霉素改良 TAP（mTAP）、含聚乙二醇的三联抗生素药物（3Mix-MP）、克林霉素改良 3Mix-MP（m3Mix-MP）、氢氧化钙（CH）和蜂胶乙醇提取物（EEP）。14 天后，去除药物，将样本提交至共聚焦激光扫描显微镜分析，以量化活菌百分比。通过 Shapiro-Wilk 检验确认数据分布。采用 Kruskal-Wallis 和 Dunn 检验进行组间比较，采用 Wilcoxon 检验比较同一药物的表面和深层抗菌效果。显著性水平设定为 P <.05。

结果

3Mix-MP 和 m3Mix-MP 与其他测试药物相比，表现出更高的抗菌效果（P <.05），除了 mTAP。mTAP 比 TAP 更有效（P <.05）。EEP 和 CH 的抗菌效果与 TAP 和 mTAP 无显著差异（P >.05）。与对照组相比，所有药物均表现出有效的抗菌作用（P <.05）。