从美国分离出的牛腺病毒7的鉴定及基因特征分析，其为腺病毒属一个新物种的假定成员。

Identification and genetic characterization of an isolate of bovine adenovirus 7 from the United States, a putative member of a new species in the genus Atadenovirus.

作者信息

Paim Willian P, Bauermann Fernando V, Kutish Gerald F, Pillatzki Angela, Long Craig, Ohnstad Martha, Diel Diego G

机构信息

Department of Veterinary Pathobiology, Oklahoma State University, 250 McElroy Hall, Stillwater, OK, 74074, USA.

Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal Do Rio Grande Do Sul (UFRGS), Porto Alegre, RS, Brazil.

出版信息

Arch Virol. 2021 Oct;166(10):2835-2839. doi: 10.1007/s00705-021-05184-x. Epub 2021 Jul 28.

DOI:10.1007/s00705-021-05184-x

PMID:34319454

Abstract

The bovine adenovirus 7 (BAdV-7) isolate SD18-74 was recovered from lung tissue of calves in South Dakota. The 30,043-nucleotide (nt) genome has the typical organization of Atadenovirus genus members. The sequence shares over 99% nt sequence identity with two Japanese BAdV-7 sequences, followed by 74.9% nt sequence identity with the ovine adenovirus 7 strain OAV287, a member of the species Ovine atadenovirus D. SD18-74 was amplified in both bovine and ovine primary nasal turbinate cells, demonstrating greater fitness in bovine cells. The genomic and biological characteristics of BAdV-7 SD18-74 support the inclusion of the members of the BAdV-7 group in a new species in the genus Atadenovirus.

摘要

牛腺病毒7型（BAdV-7）分离株SD18-74是从南达科他州犊牛的肺组织中分离得到的。其30043个核苷酸（nt）的基因组具有腺病毒属成员的典型结构。该序列与两个日本BAdV-7序列的核苷酸序列同一性超过99%，其次与绵羊腺病毒7型毒株OAV287（绵羊腺病毒D种的一个成员）的核苷酸序列同一性为74.9%。SD18-74在牛和绵羊原代鼻甲骨细胞中均能扩增，表明其在牛细胞中的适应性更强。BAdV-7 SD18-74的基因组和生物学特性支持将BAdV-7组的成员纳入腺病毒属的一个新种。