Huang Xin, Chen Qian, Li Xin, Lin Chenyu, Wang Kun, Luo Cici, Le Wenjun, Pi Xiaodong, Liu Zhongmin, Chen Bingdi
Shanghai East Hospital, The Institute for Biomedical Engineering and Nano Science, Tongji University School of Medicine, Shanghai, 200120, China.
State Key Laboratory of Silicon Materials and School of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, Zhejiang, China.
Nanoscale Res Lett. 2021 Jul 31;16(1):124. doi: 10.1186/s11671-021-03575-2.
At present, various fluorescent nanomaterials have been designed and synthesized as optical contrast agents for surgical navigation. However, there have been no reports on the preparation of fluorescent contrast agents for lung cancer surgery navigation using silicon quantum dots (Si QDs). This study improved and modified the water-dispersible Si QD micelles reported by Pi et al. to prepare Si QD micelles-CKAP4. The data showed that the Si QD micelles-CKAP4 were spherical particles with a mean hydrodiameter of approximately 78.8 nm. UV-visible absorption of the Si QD micelles-CKAP4 ranged from 200 to 500 nm. With an excitation wavelength of 330 nm, strong fluorescence at 640 nm was observed in the fluorescence emission spectra. Laser confocal microscopy and fluorescence microscopy assay showed that the Si QD micelles-CKAP4 exhibited good targeting ability to lung cancer cells and lung cancer tissues in vitro. The in vivo fluorescence-imaging assay showed that the Si QD micelles-CKAP4 was metabolized by the liver and excreted by the kidney. In addition, Si QD micelles-CKAP4 specifically targeted lung cancer tissue in vivo compared with healthy lung tissue. Cytotoxicity and hematoxylin and eosin staining assays showed that the Si QD micelles-CKAP4 exhibited high biosafety in vitro and in vivo. Si QD micelles-CKAP4 is a specifically targeted imaging agent for lung cancer and is expected to be a fluorescent contrast agent for lung cancer surgical navigation in the future.
目前，已设计并合成了各种荧光纳米材料作为手术导航的光学造影剂。然而，尚无关于使用硅量子点（Si QDs）制备用于肺癌手术导航的荧光造影剂的报道。本研究改进并修饰了Pi等人报道的水分散性Si QD胶束，制备了Si QD胶束-CKAP4。数据显示，Si QD胶束-CKAP4为球形颗粒，平均流体动力学直径约为78.8 nm。Si QD胶束-CKAP4的紫外可见吸收范围为200至500 nm。在激发波长为330 nm时，荧光发射光谱中在640 nm处观察到强荧光。激光共聚焦显微镜和荧光显微镜检测表明，Si QD胶束-CKAP4在体外对肺癌细胞和肺癌组织具有良好的靶向能力。体内荧光成像检测表明，Si QD胶束-CKAP4由肝脏代谢并经肾脏排泄。此外，与健康肺组织相比，Si QD胶束-CKAP4在体内特异性靶向肺癌组织。细胞毒性和苏木精-伊红染色检测表明，Si QD胶束-CKAP4在体外和体内均表现出高生物安全性。Si QD胶束-CKAP4是一种针对肺癌的特异性靶向成像剂，有望在未来成为肺癌手术导航的荧光造影剂。
