卵巢癌的 3D 模型。

3D Models for Ovarian Cancer.

机构信息

Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Straβe, Dresden, Germany.

Department of Chemical Engineering, Faculty of Engineering, Monash University, Melbourne, VIC, Australia.

出版信息

Adv Exp Med Biol. 2021;1330:139-149. doi: 10.1007/978-3-030-73359-9_9.

DOI:10.1007/978-3-030-73359-9_9

PMID:34339035

Abstract

The main reasons for the slow progress in improving survival outcomes for ovarian cancer are the 'one-size-fits-all' therapy and lack of clinically relevant experimental models that represent the advanced stages of the human disease. The interaction of tumour cells with their surrounding niche, the tumour microenvironment, influences the spread of ovarian cancer cells within the peritoneum and their responses to therapeutics. Scientists are increasingly using 3D cell culture models to dissect the role of the tumour microenvironment in cancer development and progression and the treatment of this disease. In this chapter, we will briefly describe the tumour microenvironment of ovarian cancer. Then, we will review some of the clinically relevant experimental approaches, such as spheroid, organoid and organotypic models, that have been developed for the 3D culture of ovarian cancer cells using different tools, including hydrogels, scaffolds and cancer-on-a-chip devices, to mimic selected components of the tumour microenvironment.

摘要

导致卵巢癌患者生存率提高进展缓慢的主要原因是“一刀切”的治疗方法，以及缺乏能够代表人类疾病晚期的临床相关实验模型。肿瘤细胞与其周围生态位（肿瘤微环境）的相互作用，影响了卵巢癌细胞在腹膜内的扩散及其对治疗的反应。科学家们越来越多地使用 3D 细胞培养模型来剖析肿瘤微环境在癌症发展和进展以及这种疾病治疗中的作用。在本章中，我们将简要描述卵巢癌的肿瘤微环境。然后，我们将回顾一些临床相关的实验方法，如球体、类器官和器官型模型，这些模型是使用不同的工具（包括水凝胶、支架和芯片上的癌症模型装置）为 3D 培养卵巢癌细胞而开发的，以模拟肿瘤微环境的选定成分。