Leung Clara, Robbins Shaina, Moss Alison, Heal Maci, Osanlouy Mahyar, Christie Richard, Farahani Navid, Monteith Corey, Chen Jin, Hunter Peter, Tappan Susan, Vadigepalli Rajanikanth, Cheng Zixi Jack, Schwaber James S
Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA.
Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA.
iScience. 2021 Jul 19;24(7):102795. doi: 10.1016/j.isci.2021.102795. eCollection 2021 Jul 23.
We developed and analyzed a single cell scale anatomical map of the rat intrinsic cardiac nervous system (ICNS) across four male and three female hearts. We find the ICNS has a reliable structural organizational plan across individuals that provide the foundation for further analyses of the ICNS in cardiac function and disease. The distribution of the ICNS was evaluated by 3D visualization and data-driven clustering. The pattern, distribution, and clustering of ICNS neurons across all male and female rat hearts is highly conserved, demonstrating a coherent organizational plan where distinct clusters of neurons are consistently localized. Female hearts had fewer neurons, lower packing density, and slightly reduced distribution, but with identical localization. We registered the anatomical data from each heart to a geometric scaffold, normalizing their 3D coordinates for standardization of common anatomical planes and providing a path where multiple experimental results and data types can be integrated and compared.
我们构建并分析了跨越四颗雄性和三颗雌性大鼠心脏的心脏固有神经系统（ICNS）单细胞尺度解剖图谱。我们发现，ICNS在个体间具有可靠的结构组织规划，为进一步分析ICNS在心脏功能和疾病中的作用奠定了基础。通过三维可视化和数据驱动聚类评估了ICNS的分布。ICNS神经元在所有雄性和雌性大鼠心脏中的模式、分布和聚类高度保守，显示出一个连贯的组织规划，其中不同的神经元簇始终定位在特定位置。雌性心脏的神经元数量较少，堆积密度较低，分布略有减少，但定位相同。我们将每颗心脏的解剖数据注册到一个几何支架上，对其三维坐标进行归一化，以标准化常见解剖平面，并提供一条可以整合和比较多个实验结果及数据类型的途径。
