囊性纤维化患者颊黏膜上皮细胞中钙调蛋白活性的改变。

Altered calmodulin activity in buccal epithelial cells from cystic fibrosis patients.

作者信息

McPherson M A, Tiligada E, Bradbury N A, Goodchild M C

机构信息

Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, UK.

出版信息

Clin Chim Acta. 1987 Dec;170(2-3):135-42. doi: 10.1016/0009-8981(87)90121-5.

DOI:10.1016/0009-8981(87)90121-5

PMID:3436048

Abstract

Boiled extracts of buccal epithelial cells from control subjects and cystic fibrosis patients were shown to possess calmodulin like activity, as assessed by their ability to activate calmodulin-deficient cyclic AMP phosphodiesterase. Estimation of calmodulin content, using pure calmodulin as standard revealed that control extracts contained 3.08 +/- 0.71 SEM (n = 7) micrograms calmodulin/mg protein and cystic fibrosis extracts 0.88 +/- 0.30 SEM (n = 12) micrograms calmodulin/mg protein (p less than 0.02 for difference from control). The results indicate that the biological activity of calmodulin is altered in buccal epithelial cells from cystic fibrosis individuals.

摘要

通过激活钙调蛋白缺陷型环磷酸腺苷磷酸二酯酶的能力评估，发现对照受试者和囊性纤维化患者的颊上皮细胞煮沸提取物具有类钙调蛋白活性。以纯钙调蛋白为标准对钙调蛋白含量进行测定，结果显示对照提取物中钙调蛋白含量为3.08±0.71 SEM（n = 7）微克/毫克蛋白质，囊性纤维化提取物中为0.88±0.30 SEM（n = 12）微克/毫克蛋白质（与对照相比差异p<0.02）。结果表明，囊性纤维化个体的颊上皮细胞中钙调蛋白的生物活性发生了改变。