Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
Int J Mol Sci. 2021 Jul 30;22(15):8198. doi: 10.3390/ijms22158198.
The insect immune response is initiated by the recognition of invading microorganisms. Peptidoglycan recognition proteins (PGRPs) function primarily as pattern recognition receptors by specifically binding to peptidoglycans expressed on microbial surfaces. We cloned a full-length cDNA for a PGRP from the Asian corn borer (Guenée) and designated it as PGRP1. mRNA was mainly detected in the fat bodies and hemocytes. Its transcript levels increased significantly upon bacterial and fungal challenges. Purified recombinant PGRP1 exhibited binding activity to the gram-positive , gram-negative , entomopathogenic fungi , and yeast The binding further induced their agglutination. Additionally, PGRP1 preferred to bind to Lys-type peptidoglycans rather than DAP-type peptidoglycans. The addition of recombinant PGRP1 to plasma resulted in a significant increase in phenoloxidase activity. The injection of recombinant PGRP1 into larvae led to a significantly increased expression of several antimicrobial peptide genes. Taken together, our results suggest that PGRP1 potentially recognizes the invading microbes and is involved in the immune response in .
昆虫的免疫反应是由识别入侵的微生物引发的。肽聚糖识别蛋白（PGRPs）主要作为模式识别受体，通过特异性结合微生物表面表达的肽聚糖发挥作用。我们从亚洲玉米螟（Guenée）中克隆了一个全长的 PGRP cDNA，并将其命名为 PGRP1。PGRP1 mRNA 主要在脂肪体和血细胞中检测到。其转录水平在细菌和真菌挑战后显著增加。纯化的重组 PGRP1 表现出与革兰氏阳性菌、革兰氏阴性菌、昆虫病原真菌和酵母的结合活性。这种结合进一步诱导了它们的聚集。此外，PGRP1 更喜欢结合 Lys 型肽聚糖，而不是 DAP 型肽聚糖。重组 PGRP1 加入到血浆中会导致酚氧化酶活性显著增加。将重组 PGRP1 注射到幼虫中会导致几种抗菌肽基因的表达显著增加。综上所述，我们的研究结果表明，PGRP1 可能识别入侵的微生物，并参与亚洲玉米螟的免疫反应。
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