Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura,35516, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura,35516, Egypt; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jan 5;264:120241. doi: 10.1016/j.saa.2021.120241. Epub 2021 Jul 29.
A new approach to determine the local anesthetic benoxinate spectrofluorimetrically was developed. It was found that benoxinate exhibits strong native fluorescence in ethanol at 371 nm after excitation at 297 nm. There was a linear response between the fluorescence intensity and the concentration of the studied drug over the range of 10.0-100.0 ng/ mL. The suggested spectrofluorimetric method was optimized and validated following the pharmacopoeial guidelines. The obtained results were fully discussed and statistically analyzed in relevance to a previous published spectroscopic method. The limit of quantification (LOQ) of the present method was 1.79 ng/mL. Inter-day and intra-day precision relative standard deviations were lower than 1.5%.Finally, the proposed methodology has been adopted for determination of benoxinate in aqueous humor with a mean percentage recovery 99.87 ± 1.66 as well as in the pharmaceutical eye drops with a mean percentage recovery 100.37 ± 1.32. The method is cost-effective and green as it depends in water and ethanol mainly.
开发了一种新的方法，通过荧光光谱法来确定局部麻醉药苯佐卡因。研究发现，苯佐卡因在乙醇中于 371nm 处激发，在 297nm 处显示出强烈的固有荧光。在 10.0-100.0ng/mL 的范围内，荧光强度与研究药物的浓度之间存在线性响应。该建议的荧光分光光度法是根据药典指南进行优化和验证的。对所得结果进行了充分的讨论和统计分析，并与以前发表的分光光度法进行了比较。本方法的定量限（LOQ）为 1.79ng/mL。日内和日间精密度相对标准偏差均低于 1.5%。最后，采用该方法测定了房水中的苯佐卡因，平均回收率为 99.87%±1.66%，以及在眼科制剂中的平均回收率为 100.37%±1.32%。该方法具有成本效益和环保效益，因为它主要依赖于水和乙醇。
Zotero 插件