小鼠肾脏中的神经生长因子免疫反应性：一项免疫电子显微镜研究。

Nerve growth factor immunoreactivity in mouse kidney: an immunoelectron microscopic study.

作者信息

Barajas L, Salido E C, Laborde N P, Fisher D A

机构信息

Department of Pathology, Harbor-UCLA Medical Center, Torrance 90509.

出版信息

J Neurosci Res. 1987;18(3):418-24. doi: 10.1002/jnr.490180306.

DOI:10.1002/jnr.490180306

PMID:3437465

Abstract

We have recently reported the immunolocalization of nerve growth factor (NGF) in mouse kidney by light microscopy. In the present study, we have investigated the ultrastructural localization of NGF by the preembedding immunoperoxidase method for electron microscopy. NGF immunoreactivity was present in the connecting tubule cells of the distal nephron. These cells showed immunostaining associated with the Golgi complex, vesicles, rough endoplasmic reticulum (RER), and polyribosomes. The intercalated cells, in contrast, lacked immunoreactivity.

摘要

我们最近通过光学显微镜报道了神经生长因子（NGF）在小鼠肾脏中的免疫定位。在本研究中，我们采用用于电子显微镜的包埋前免疫过氧化物酶法研究了NGF的超微结构定位。NGF免疫反应性存在于远端肾单位的连接小管细胞中。这些细胞显示出与高尔基体、囊泡、粗面内质网（RER）和多聚核糖体相关的免疫染色。相比之下，闰细胞缺乏免疫反应性。