Imbalance between the circulating endothelium-derived apoptotic microparticles and the endothelial colony-forming units of progenitor cells in patients undergoing diagnostic coronary angiography.

PURPOSE

The involvement of the circulating endothelium-derived microparticles (EMPs) and the endothelial progenitor cells (EPCs) has been shown in the pathogenesis of coronary artery disease (CAD). The current study aimed to explore whether the Friesinger index is associated with the levels of the apoptotic CD144+/CD31+/annexin V+ ​EMPs and the number of endothelial colony-forming units of progenitor cells in patients undergoing coronary angiography.

PATIENTS AND METHODS

Fifty-seven patients with a median age of 62 years (range: 48-84 years) were enrolled. Quantification of the apoptotic CD144/CD31/annexin V EMPs was performed by flow cytometry. The number of endothelial colony-forming units defined by CFU-Hill was assessed by cell culture.

RESULTS

There was a positive correlation between the Friesinger index and the circulating levels of the apoptotic CD144/CD31/annexin V EMPs (rho=0.817, p<0.001), whereas a negative correlation was found with the number of CFU-Hill (rho ​= ​- 0.649, p<0.001). Multivariable logistic analysis showed that the risk of having moderate/severe CAD was five times greater among male patients (OR:5.32; 95% CI: 1.19 - 16.33; p=0.038) and almost one and a half times higher among those with a higher level of apoptotic CD144/CD31/annexin V EMPs (OR:1.74; 95% CI: 1.23 - 2.28; p=0.001). Finally, the circulating levels of apoptotic EMPs labelled for CD144/CD31/annexin V presented a good discrimination of moderate/severe CAD, with an AUC of 0.85 (95% CI ​= ​0.74 - 0.96; p< 0.001).

CONCLUSIONS

Moderate or severe CAD is associated with increased levels of apoptotic EMPs and reduced EPC colony-forming capacity, increasing the occurrence of endothelial injuries.