First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Nursing College, Nanjing University of Chinese Medicine, Nanjing 210023, China.
J Tradit Chin Med. 2021 Aug;41(4):564-570. doi: 10.19852/j.cnki.jtcm.2021.03.008.
To explore the therapeutic effect and inflammatory mechanism of fire needles on gouty arthritis.
Sixty male Sprague-Dawley rats were divided into four groups, that is, control group, the model group, the colchicine group, and the fire needle treatment group. Acute gouty arthritis was prepared by injection of monosodium urate in the ankle joint. The inflammation-related protein [toll-like receptor 4 (TLR4), pyrin domain-containing protein 3 (NLRP3), interleukin (IL)-1β, myeloid differentiation factor 88 (MyD88), nuclear factorkappa B (NF-κB), phosphorylated NF-κB (p-NF-κB), caspase-1, and p-caspase-1] in swollen tissues, the inflammation-related mRNAs indicators (TLR4, NLRP3, NF-κB, and caspase-1) and the inflammatory factors [IL-1β, IL-6, IL-8, tumor necrosis factor-α (TNF-α), and C-reactive protein (CRP)] in the serum were detected by Western blot, real-time quantitative polymerase chain reaction, and enzyme-linked immunosorbent assay, respectively.
The fire needle treatment could reduce joint swelling, increase mechanical pain threshold and decrease the inflammation index score in the fire needle treatment group. It could also significantly decreased the protein expression of IL-1β, TLR4, MyD88, p-NF-κB and NLRP3 in joint tissue, markedly downregulated the mRNA levels of TLR4 and NLRP3 in joint tissue, and significantly reduce serum levels of IL-1β, IL-6, IL-8, TNF-α, and CRP.
The fire needle treatment had positive effect of treating gouty arthritis, and its under- lying mechanism might be associated with NF-κB activation and related inflammatory response.
探讨火针对痛风性关节炎的治疗作用及炎症机制。
将 60 只雄性 Sprague-Dawley 大鼠随机分为 4 组，即对照组、模型组、秋水仙碱组和火针组。通过踝关节注射单钠尿酸盐制备急性痛风性关节炎。采用 Western blot 法检测肿胀组织中炎症相关蛋白[Toll 样受体 4（TLR4）、含pyrin 结构域蛋白 3（NLRP3）、白细胞介素（IL）-1β、髓样分化因子 88（MyD88）、核因子-κB（NF-κB）、磷酸化 NF-κB（p-NF-κB）、半胱氨酸天冬氨酸蛋白酶-1（caspase-1）和 p-caspase-1]，实时定量聚合酶链反应法检测肿胀组织中炎症相关 mRNAs 指标（TLR4、NLRP3、NF-κB 和 caspase-1），酶联免疫吸附法检测血清中炎症因子[IL-1β、IL-6、IL-8、肿瘤坏死因子-α（TNF-α）和 C 反应蛋白（CRP）]。
火针治疗可减轻关节肿胀，提高机械痛阈，降低火针治疗组炎症指数评分。火针治疗还可显著降低关节组织中 IL-1β、TLR4、MyD88、p-NF-κB 和 NLRP3 的蛋白表达，显著下调关节组织中 TLR4 和 NLRP3 的 mRNA 水平，显著降低血清中 IL-1β、IL-6、IL-8、TNF-α 和 CRP 的水平。
火针治疗痛风性关节炎有积极作用，其作用机制可能与 NF-κB 激活及相关炎症反应有关。
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