母鼠分离诱导抑郁样子代大鼠肠道氨基酸途径紊乱。
Disorder of intestinal amino acid pathway in depression-like offspring rats induced by maternal separation.
机构信息
Section of Scientific Research, the First Affiliated Hospital of Xi’an Medical College, Xi’an 710077, China
Preparation Center, Xi’an Daxing Hospital, Xi’an 710076, China
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2021 Jun 25;50(3):298-304. doi: 10.3724/zdxbyxb-2021-0102.
To investigate the intestinal amino acids pathway in depression-like offspring rats induced by maternal separation. Sprague-Dawley (SD) female rats were randomly divided into a control group (=8) and a maternal separation group (=8). After normal delivery, the maternal rats were separated from offsprings for 14 consecutive days and 3 h per day in maternal separation group; while rats in the control group was received no interventions in postpartum. Depression-like behaviors of offspring rats were evaluated using the sucrose preference test, novelty suppressed feeding test, and forced swimming test. Amino acid analyzer was used to detect the changes of amino acid contents in the small intestine, and the expressions of alanine-serine-cysteine transporter 2 (ASCT2), solute carrier superfamily 6 member 19 (BAT1) and L-type amino acid transporter 1(LAT1) were detected by Western blot. The weight of the offspring rats in the maternal separation group was significantly lower than that of the control group at 21 and 28 d (=4.925 and 5.766, all <0.01). Compared with the control group, the percentage of sucrose preference of the offspring rats in the maternal separation group was significantly reduced (=2.709, <0.05), and the feeding latency was significantly prolonged (=-13.431, <0.01). The immobility time in FST of maternal separation group was significantly longer (=-3.616, <0.01).Increased concentration of aspartic acid (=-6.672, <0.01) and down-regulation of glutamine (=3.107, <0.01) and glycine (=9.781, <0.01) were observed in maternal separation group. Western blot analysis revealed that the protein expressions of ASCT2 (=6.734, <0.01) and BAT1 (=9.015, <0.01) in maternal separation group were reduced, while the expression of LAT1 was increased (=-8.942, <0.01). Maternal separation can induce the depression-like behavior in offspring rats; the amino acid contents and the amino acid transporter expression in the small intestine are reduced, which may be related to depression-like behavior induced by maternal separation.
为了研究母体分离诱导的抑郁样后代大鼠的肠道氨基酸途径。将 Sprague-Dawley (SD) 雌性大鼠随机分为对照组（=8）和母体分离组（=8）。母体分离组在产后连续 14 天每天分离后代 3 小时；而对照组的大鼠在产后没有接受任何干预。通过蔗糖偏好试验、新异抑制进食试验和强迫游泳试验评估后代大鼠的抑郁样行为。氨基酸分析仪检测小肠氨基酸含量变化，Western blot 检测丙氨酸-丝氨酸-半胱氨酸转运体 2 (ASCT2)、溶质载体家族 6 成员 19 (BAT1) 和 L 型氨基酸转运体 1 (LAT1) 的表达。母体分离组后代大鼠体重在 21 和 28 天明显低于对照组（=4.925 和 5.766，均<0.01）。与对照组相比，母体分离组后代大鼠的蔗糖偏好百分比明显降低（=2.709，<0.05），进食潜伏期明显延长（=-13.431，<0.01）。母体分离组 FST 的不动时间明显延长（=-3.616，<0.01）。母体分离组观察到天冬氨酸浓度升高（=-6.672，<0.01），谷氨酰胺（=-3.107，<0.01）和甘氨酸（=-9.781，<0.01）下调。Western blot 分析显示，母体分离组 ASCT2（=6.734，<0.01）和 BAT1（=9.015，<0.01）的蛋白表达减少，而 LAT1 的表达增加（=-8.942，<0.01）。母体分离可诱导后代大鼠出现抑郁样行为；小肠氨基酸含量和氨基酸转运体表达降低，可能与母体分离诱导的抑郁样行为有关。
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