长期摄入 - 补充饮食可增强大鼠海马体的神经认知功能，抑制氧化应激、乙酰胆碱酯酶活性和神经元退行性变。
Long-term consumption of -supplemented diet enhanced neurocognition, suppressed oxidative stress, acetylcholinesterase activity and neuronal degeneration in rat's hippocampus.
机构信息
Neurophysiology Unit, Department of Physiology, PAMO University of Medical Sciences, Port-Harcourt, River State, Nigeria.
Department of Biochemistry, Faculty of Basic Medical Sciences, PAMO University of Medical Sciences, Port-Harcourt, Nigeria.
出版信息
Drug Metab Pers Ther. 2021 Mar 26;36(3):223-231. doi: 10.1515/dmpt-2020-0189.
OBJECTIVES
This study investigates protection against oxidative stress and memory enhancing potential of long-term consumption of leaves.
METHODS
Male Wistar rat were fed with mixture of -supplemented diets (MOSD) partitioned in 1, 5, 10, and 20% continuously for 12 weeks. Object recognition test (ORT) and Morris water maze (MWM) was used for assessing neurocognition. Changes in body weight, Lipid peroxidation (MDA), Glutathione (GSH), Catalase (CAT) and Acetylcholinesterase (AChE) activity was assayed in the brain tissue. Histomorphometric of the hippocampus was also examined.
RESULTS
The diets progressively increase the body weigh after the 12 weeks, improved spatial (MWM) and non-spatial (ORT) memory performance, protect against oxidative stress, inhibit AChE activity and suppresses neuronal degeneration in the hippocampus when stained with Cresyl violent stain.
CONCLUSIONS
Conclusively, long-term consumption of MOSD shows strong protection against oxidative stress and hippocampal degeneration and improves neurocognition with dose dependent effect.
目的
本研究旨在探讨长期摄入叶子对氧化应激的保护作用和增强记忆的潜力。
方法
雄性 Wistar 大鼠连续 12 周喂食含 -的混合饮食（MOSD），分为 1%、5%、10%和 20%四组。采用物体识别测试（ORT）和 Morris 水迷宫（MWM）评估神经认知功能。检测脑组织中体重变化、脂质过氧化（MDA）、谷胱甘肽（GSH）、过氧化氢酶（CAT）和乙酰胆碱酯酶（AChE）活性的变化。还对海马体进行了组织形态计量学检查。
结果
12 周后，饮食逐渐增加体重，改善空间（MWM）和非空间（ORT）记忆表现，抵抗氧化应激，抑制乙酰胆碱酯酶活性，抑制 Cresyl 暴力染色后海马体的神经元变性。
结论
总之，长期摄入 MOSD 显示出对氧化应激和海马体变性的强烈保护作用，并具有剂量依赖性的神经认知改善作用。
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