Wageningen UR Plant Breeding, Wageningen University and Research, Wageningen, The Netherlands.
Methods Mol Biol. 2021;2354:303-313. doi: 10.1007/978-1-0716-1609-3_14.
Late blight in potato, caused by the oomycete Phytophthora infestans, is a devastating disease that significantly impacts potato production. For a proper understanding of disease development, it is important to understand the interaction between plant and pathogen at a molecular level. Like other pathogens, P. infestans secretes effector molecules, which can be recognized by receptors in the plant and trigger immunity. In addition, effectors from P. infestans have been identified to enhance disease development. Here, we describe an assay to investigate the role of effectors in virulence of P. infestans on potato. We combine agroinfiltration to transiently express effectors in potato with detached leaf assays to monitor disease development. This protocol makes it possible to conveniently quantify the effect of individual effectors on virulence of P. infestans. The identification of effectors with an important role in late blight development can help to design better strategies to control the disease.
晚疫病是由卵菌 Phytophthora infestans 引起的马铃薯病害，它是一种严重影响马铃薯生产的破坏性疾病。为了正确理解疾病的发展，了解植物和病原体在分子水平上的相互作用非常重要。像其他病原体一样，P. infestans 会分泌效应子分子，这些分子可以被植物中的受体识别，并引发免疫反应。此外，还鉴定出 P. infestans 的效应子可以增强疾病的发展。在这里，我们描述了一种用于研究 P. infestans 效应子在马铃薯致病力中作用的测定方法。我们将效应子的瞬时表达与离体叶片测定相结合，以监测疾病的发展。该方案可方便地量化单个效应子对 P. infestans 致病力的影响。鉴定在晚疫病发展中起重要作用的效应子有助于设计更好的控制疾病的策略。
