嘌呤能药物对高钾诱导的大鼠海马切片癫痫样爆发的调节作用。

Modulatory action of purinergic drugs on high potassium-induced epileptiform bursting in rat hippocampal slices.

作者信息

Sagratella S, Frank C, Benedetti M, Scotti de Carolis A

机构信息

Laboratorio di Farmacologia, Istituto Superiore di Sanità, Roma, Italy.

出版信息

Pharmacol Res Commun. 1987 Nov;19(11):819-26. doi: 10.1016/0031-6989(87)90015-4.

DOI:10.1016/0031-6989(87)90015-4

PMID:3444843

Abstract

Increase of the potassium concentration up to 8 mM in the superfused solution of rat hippocampal slices leads to the development of an epileptiform bursting. The derivative agonist L-phenyl isopropyl adenosine (L-PIA) (0.05-0.5 microM) is able to block the potassium induced epileptiform activity. The adenosine antagonist caffeine (100 microM) reverts the antiepileptic effect of L-PIA. Our data show a modulatory action of the purinergic transmission in a model of experimental in vitro epilepsy, and point out about a control of endogenous adenosine in the development of focal epileptiform activity. The relationships between the purinergic influence on the release of neurotransmitters, and the convulsant-anticonvulsant effects of the drugs are discussed.

摘要

将大鼠海马切片灌流溶液中的钾浓度提高到8 mM会导致癫痫样爆发的发生。衍生激动剂L-苯基异丙基腺苷（L-PIA）（0.05 - 0.5 microM）能够阻断钾诱导的癫痫样活动。腺苷拮抗剂咖啡因（100 microM）可逆转L-PIA的抗癫痫作用。我们的数据显示了嘌呤能传递在体外实验性癫痫模型中的调节作用，并指出内源性腺苷在局灶性癫痫样活动发展中的控制作用。还讨论了嘌呤能对神经递质释放的影响与药物惊厥 - 抗惊厥作用之间的关系。