增强的双分子层体性质通过增加酸化途径产生了最佳药理作用。
Enhanced Bilosomal Properties Resulted in Optimum Pharmacological Effects by Increased Acidification Pathways.
作者信息
Mooranian Armin, Foster Thomas, Ionescu Corina M, Walker Daniel, Jones Melissa, Wagle Susbin Raj, Kovacevic Bozica, Chester Jacqueline, Johnston Edan, Wong Elaine, Atlas Marcus D, Mikov Momir, Al-Salami Hani
机构信息
The Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, Australia.
Hearing Therapeutics, Ear Science Institute Australia, Queen Elizabeth II Medical Centre, Perth, WA 6009, Australia.
出版信息
Pharmaceutics. 2021 Jul 31;13(8):1184. doi: 10.3390/pharmaceutics13081184.
INTRODUCTION
Recent studies in our laboratory have shown that some bile acids, such as chenodeoxycholic acid (CDCA), can exert cellular protective effects when encapsulated with viable β-cells via anti-inflammatory and anti-oxidative stress mechanisms. However, to explore their full potential, formulating such bile acids (that are intrinsically lipophilic) can be challenging, particularly if larger doses are required for optimal pharmacological effects. One promising approach is the development of nano gels. Accordingly, this study aimed to examine biological effects of various concentrations of CDCA using various solubilising nano gel systems on encapsulated β-cells.
METHODS
Using our established cellular encapsulation system, the Ionic Gelation Vibrational Jet Flow technology, a wide range of CDCA β-cell capsules were produced and examined for morphological, biological, and inflammatory profiles.
RESULTS AND CONCLUSION
Capsules' morphology and topographic characteristics remained similar, regardless of CDCA or nano gel concentrations. The best pharmacological, anti-inflammatory, and cellular respiration, metabolism, and energy production effects were observed at high CDCA and nano gel concentrations, suggesting dose-dependent cellular protective and positive effects of CDCA when incorporated with high loading nano gel.
引言
我们实验室最近的研究表明，某些胆汁酸，如鹅去氧胆酸（CDCA），当与活的β细胞封装在一起时，可通过抗炎和抗氧化应激机制发挥细胞保护作用。然而，为了探索它们的全部潜力，配制这类本质上具有亲脂性的胆汁酸可能具有挑战性，特别是如果需要更大剂量才能达到最佳药理效果。一种有前景的方法是开发纳米凝胶。因此，本研究旨在使用各种增溶纳米凝胶系统，研究不同浓度的CDCA对封装的β细胞的生物学效应。
方法
使用我们已建立的细胞封装系统，即离子凝胶化振动喷射流技术，制备了一系列CDCAβ细胞胶囊，并对其形态、生物学和炎症特征进行了检测。
结果与结论
无论CDCA或纳米凝胶浓度如何，胶囊的形态和地形特征保持相似。在高CDCA和纳米凝胶浓度下观察到最佳的药理、抗炎以及细胞呼吸、代谢和能量产生效应，这表明当与高负载纳米凝胶结合时，CDCA具有剂量依赖性的细胞保护和积极作用。
Zotero 插件