免疫组化和 qPCR 检测 SARS-CoV-2 在人类中耳与鼻腔中的分布：病例系列研究。
Immunohistochemical and qPCR Detection of SARS-CoV-2 in the Human Middle Ear Versus the Nasal Cavity: Case Series.
机构信息
Department of Surgery/ Division of Otolaryngology- Head and Neck Surgery, University of California, San Diego, USA.
University of California San Diego, School of Medicine, 9500 Gilman Drive, La Jolla, CA, 92093-0666, USA.
出版信息
Head Neck Pathol. 2022 Jun;16(2):607-611. doi: 10.1007/s12105-021-01378-6. Epub 2021 Aug 28.
Viral infections have already been implicated with otitis media and sudden sensorineural hearing loss. However, the pathophysiology of COVID-19 as it relates to otologic disorders is not well-defined. With the spread of SARS-CoV-2, it is important to evaluate its colonization of middle ear mucosa. Middle ear and nasal tissue samples for quantitative RT-PCR and histologic evaluations were obtained from post-mortem COVID-19 patients and non-diseased control patients. Here we present evidence that SARS-CoV-2 colonizes the middle ear epithelium and co-localizes with the primary viral receptor, angiotensin-converting enzyme 2 (ACE2). Both middle ear and nasal epithelial cells show relatively high expression of ACE2, required for SARS-CoV-2 entry. The epithelial cell adhesion molecule (EpCAM) was use as a biomarker of epithelia. Furthermore, we found that the viral load in the middle ear is lower than that present in the nasal cavity.
病毒感染已被认为与中耳炎和突发性感觉神经性听力损失有关。然而，COVID-19 与耳科疾病相关的病理生理学尚未明确。随着 SARS-CoV-2 的传播，评估其对中耳黏膜的定植情况很重要。从中耳和鼻腔组织中提取定量 RT-PCR 和组织学评估样本，取自 COVID-19 患者和非疾病对照患者的尸体。在此，我们提供的证据表明，SARS-CoV-2 定植于中耳上皮细胞，与主要病毒受体血管紧张素转换酶 2（ACE2）共定位。中耳和鼻腔上皮细胞均表现出相对较高的 ACE2 表达，这是 SARS-CoV-2 进入细胞所需的。上皮细胞黏附分子（EpCAM）被用作上皮细胞的生物标志物。此外，我们发现中耳中的病毒载量低于鼻腔中的病毒载量。
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