用于酵母和鸡肝H3蛋白酶的组蛋白H3切割分析
Histone H3 Cleavage Assay for Yeast and Chicken Liver H3 Protease.
作者信息
Chauhan Sakshi, Azad Gajendra Kumar, Tomar Raghuvir Singh
机构信息
Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India.
Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel.
出版信息
Bio Protoc. 2017 Jan 5;7(1):e2085. doi: 10.21769/BioProtoc.2085.
Histone proteins are subjected to a wide array of reversible and irreversible post-translational modifications (PTMs) (Bannister and Kouzarides, 2011; Azad and Tomar, 2014). The PTMs on histones are known to regulate chromatin structure and function. Histones are irreversibly modified by proteolytic clipping of their tail domains. The proteolytic clipping of histone tails is continuously attracting interest of researchers in the field of chromatin biology. We can recapitulate H3-clipping by performing H3 cleavage assay. Here, we are presenting the detailed protocol to perform H3 cleavage assay.
组蛋白会经历一系列广泛的可逆和不可逆的翻译后修饰（PTMs）（班尼斯特和库扎里德斯，2011年；阿扎德和托马尔，2014年）。已知组蛋白上的PTMs可调节染色质的结构和功能。组蛋白通过其尾部结构域的蛋白水解剪切而发生不可逆修饰。组蛋白尾部的蛋白水解剪切一直吸引着染色质生物学领域研究人员的关注。我们可以通过进行H3剪切试验来重现H3剪切过程。在此，我们展示进行H3剪切试验的详细方案。
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