Soffan Alan, Subandiyah Siti, Wijonarko Arman, Sawitri Widhi Dyah
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Research Center for Biotechnology, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Data Brief. 2021 Aug 16;38:107302. doi: 10.1016/j.dib.2021.107302. eCollection 2021 Oct.
Tea Mosquito Bug (TMB), (Hemiptera: Miridae) is one of the major pest infesting tea and cocoa plantations worldwide. Developing olfaction-based control methods was urges as an alternative to commonly used but non-environmental friendly chemical pesticides. However, the molecular mechanisms underlying TMB reception mechanism are still lacking. Here, we collected a pooled male and female TMB antennae for RNA extraction followed by sequencing using the BGISEQ-500 platform and de novo assembly. TMB antennae RNA-seq data yielded 32,142 unigenes with N50 and GC (%) were 2322 and 40.25; subsequently. The RNA-seq data are available in GenBank Sequence Read Archive (SRA) database with accession number SRR13327229. De novo transcriptome analysis had identified several genes involved in TMB odorant reception includes; 39 OBPs (odorant binding proteins), 10 CSPs (chemosensory proteins), 81 Ors (odorant receptors), 1 Orcos (co-receptors), 9 SNMPs (sensory neuron membrane proteins), 3 GRs (gustatory receptors) and 4 IRs (ionotropic receptors). Our study presents the first RNA seq for TMB antennae, which serve the primary molecular resources data, which will facilitate further research to develop olfaction-based control methods, potentially contribute to TMB management strategies.
茶小绿叶蝉(半翅目:盲蝽科)是全球茶和可可种植园中主要的害虫之一。由于常用的化学农药不环保,因此迫切需要开发基于嗅觉的防治方法。然而,茶小绿叶蝉嗅觉接收机制的分子机制仍然缺乏。在这里,我们收集了混合的茶小绿叶蝉雌雄触角用于RNA提取,然后使用BGISEQ-500平台进行测序并从头组装。茶小绿叶蝉触角RNA-seq数据产生了32,142个单基因,N50和GC(%)分别为2322和40.25;随后。RNA-seq数据可在GenBank序列读取存档(SRA)数据库中获得,登录号为SRR13327229。从头转录组分析鉴定了几个参与茶小绿叶蝉气味接收的基因,包括;39个气味结合蛋白(OBP)、10个化学感受蛋白(CSP)、81个气味受体(Or)、1个共同受体(Orcos)、9个感觉神经元膜蛋白(SNMP)、3个味觉受体(GR)和4个离子型受体(IR)。我们的研究展示了首个茶小绿叶蝉触角RNA测序,它提供了主要的分子资源数据,这将有助于进一步研究开发基于嗅觉的防治方法,可能有助于茶小绿叶蝉的管理策略。