应用QuantiFERON ELISA检测成人起病型免疫缺陷综合征中的干扰素-γ自身抗体。

Application of QuantiFERON ELISA for Detection of Interferon-Gamma Autoantibodies in Adult-Onset Immunodeficiency Syndrome.

作者信息

Khositnithikul Rommanee, Laisuan Wannada, Setthaudom Chavachol, Sriwanichrak Kanchana, Kunakorn Mongkol, Srikhirin Toemsak, Lumjiaktase Putthapoom, Vongsakulyanon Apirom

机构信息

Clinical Pathology Programme, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

出版信息

Lab Med. 2022 Jan 6;53(1):12-17. doi: 10.1093/labmed/lmab039.

DOI:10.1093/labmed/lmab039

PMID:34463313

Abstract

OBJECTIVE

Patients who develop interferon-gamma autoantibodies (IFN-ɤ autoAbs) in adult-onset immunodeficiency (AOID) syndrome are more likely to develop opportunistic and recurrent intracellular infections. The assay to detect IFN-ɤ autoAbs is essential for the diagnosis and therapeutic monitoring of AOID syndrome. Therefore, this study applied the QuantiFERON assay for the detection of IFN-ɤ autoAbs.

METHODS

Serum from patients with AOID syndrome (n = 19) and serum from healthy patients (n = 20) was collected and applied using 2 neutralizing platforms of enzyme-linked immunosorbent assay (ELISA) kits (the BD ELISA and the QuantiFERON ELISA) for IFN-ɤ autoAbs detection.

RESULTS

The pooled serum from patients with AOID syndrome showed >50% inhibition at 1:5000 dilution (positive), whereas the pooled serum from healthy patients showed <50% inhibition at 1:5000 dilution (negative) according to the neutralizing QuantiFERON ELISA. Each specimen showed the same result according to both the neutralizing BD ELISA and the neutralizing QuantiFERON ELISA. Moreover, the patient serum showed a variation in titer ranging from 1:5000 to >1:5,000,000 according to the neutralizing QuantiFERON ELISA.

CONCLUSION

The QuantiFERON ELISA kit could be applied for the detection of IFN-ɤ autoAbs for the diagnosis and therapeutic monitoring of AOID syndrome.

摘要

目的

在成人起病免疫缺陷（AOID）综合征中出现干扰素-γ自身抗体（IFN-ɤ自身抗体）的患者更易发生机会性和复发性细胞内感染。检测IFN-ɤ自身抗体的检测方法对于AOID综合征的诊断和治疗监测至关重要。因此，本研究应用QFT检测法检测IFN-ɤ自身抗体。

方法

收集AOID综合征患者（n = 19）和健康患者（n = 20）的血清，并使用2种酶联免疫吸附测定（ELISA）试剂盒的中和平台（BD ELISA和QFT ELISA）检测IFN-ɤ自身抗体。

结果

根据中和QFT ELISA，AOID综合征患者的混合血清在1:5000稀释度时显示>50%抑制（阳性），而健康患者的混合血清在1:5000稀释度时显示<50%抑制（阴性）。根据中和BD ELISA和中和QFT ELISA，每个样本均显示相同结果。此外，根据中和QFT ELISA，患者血清的滴度在1:5000至>1:5,000,000之间变化。