LHPP 表达下调与 AFP 相关，可作为人肝癌的一个良好预后因素。
Downregulation of LHPP Expression Associated with AFP Acts as a Good Prognostic Factor in Human Hepatocellular Carcinoma.
机构信息
The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China.
The College of Basic Medicine Sciences, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
出版信息
Biomed Res Int. 2021 Aug 25;2021:1971048. doi: 10.1155/2021/1971048. eCollection 2021.
BACKGROUND
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) serves as a tumor suppressor in hepatocellular carcinoma (HCC), but the correlation between the expression of LHPP and the clinical parameters of oncogenic progression is still not well defined. This study is to reveal the correlation between the expression of LHPP in HCC and their clinical parameters.
METHODS
Immunohistochemical analysis was used to assess the correlation between the expression of LHPP and the clinical parameters of HCC. Expressions of LHPP in HCC tissues and cultured HCC cells were detected by Western blot and quantitative real-time polymerase chain reaction (qRT-PCR). LHPP, gamma-glutamyl transferase (GGT), and -fetoprotein (AFP) expression levels in blood or HCC tissues were detected by enzyme-linked immunosorbent assay (ELISA). The Spearman rank correlation coefficient was used to evaluate the correlation of the expression of LHPP and the clinical index of HCC. Correlation of survival and expression of LHPP were analyzed using the Kaplan-Meier method and the log-rank test.
RESULTS
Expressions of LHPP in HCC tissues were significantly downregulated than their paired adjacent normal tissues. A significant positive correlation was found between the cytoplasm and nuclear expression of LHPP in both HCC and their paired adjacent normal tissues. The expression of LHPP negatively correlated with the levels of GGT in the cytoplasm of adjacent tissues and with the AFP level in the nucleus of HCC cells. Relative levels of LHPP in HCC tissues were markedly lower than those of the paired adjacent normal tissues. Relative levels of LHPP in LO-2 cells were higher than those of HepG2, BEL-7404, and SMMC-7721 cell lines. The overall survival and DSF survival of patients with the high expression of LHPP were much higher than those with the low expression of LHPP in paired adjacent normal tissue.
CONCLUSIONS
LHPP is associated with the AFP level and acts as a good prognostic factor in HCC.
背景
磷酸丝氨酸磷酸组氨酸无机焦磷酸酶 (LHPP) 作为肝细胞癌 (HCC) 的肿瘤抑制因子，但 LHPP 的表达与致癌进展的临床参数之间的相关性尚不清楚。本研究旨在揭示 LHPP 在 HCC 中的表达与临床参数之间的相关性。
方法
采用免疫组织化学分析评估 LHPP 表达与 HCC 临床参数之间的相关性。采用 Western blot 和实时定量聚合酶链反应 (qRT-PCR) 检测 HCC 组织和培养的 HCC 细胞中 LHPP 的表达。采用酶联免疫吸附试验 (ELISA) 检测血液或 HCC 组织中 LHPP、γ-谷氨酰转移酶 (GGT) 和 -甲胎蛋白 (AFP) 的表达水平。采用 Spearman 秩相关系数评估 LHPP 表达与 HCC 临床指标的相关性。采用 Kaplan-Meier 方法和对数秩检验分析 LHPP 表达与生存的相关性。
结果
与配对的相邻正常组织相比，HCC 组织中 LHPP 的表达明显下调。在 HCC 及其配对的相邻正常组织中，LHPP 的细胞质和核表达均呈显著正相关。LHPP 的表达与相邻组织细胞质中 GGT 的水平以及 HCC 细胞核中 AFP 的水平呈负相关。与配对的相邻正常组织相比，HCC 组织中 LHPP 的相对水平明显降低。LO-2 细胞中 LHPP 的相对水平明显高于 HepG2、BEL-7404 和 SMMC-7721 细胞系。在配对的相邻正常组织中，LHPP 高表达患者的总生存率和 DSF 生存率明显高于 LHPP 低表达患者。
结论
LHPP 与 AFP 水平相关，是 HCC 的一个良好预后因素。
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