College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
Experimental and Research Animal Institute, Sichuan University, Chengdu 610065, People's Republic of China.
Biomed Mater. 2021 Oct 4;16(6). doi: 10.1088/1748-605X/ac2492.
Aseptic loosening of artificial joints is the most common complication after artificial joint replacement. Finding the solution to tackle aseptic loosening of artificial joints is a focus in bone and joint surgery research field.studies of Sr-doped calcium polyphosphate (SCPP) have found by our team that it could promote osteoblast proliferation and inhibit osteoclast activity, and it has a potential inhibitory effect on aseptic loosening by suppressing the expression of receptor activator of nuclear factor-κ B ligand and improving the expression of OPG. The present study aims to confirm the conclusionby the mean of animal experiment. The Ti rod prosthesis coated with SCPP, calcium polyphosphate (CPP), and Ultra-high molecular weight polyethylene (UHMWPE were implanted in the femur (the internal surface of bone tunnel was also coated with SCPP, CPP and UHMWPE respectively). Radiography (x-rays, micro-CT), histochemistry (Hematoxylin-eosin staining (HE), methylene blue-acid fuchsin, Von Kossa histological staining), molecular biology (alkaline phosphatase and TRAP5b factors, Mir21-5p and Mir 26a-5p) were performed to analyzed the effects of SCPP within 20 weeks. The Radiography results showed that osteolysis with various severity occurred in all groups, and SCPP group had the mildest osteolysis. Histochemistry results showed that arthritis was milder in SCPP and CPP groups, while the bone formation in SCPP group was most significant. Its bone reconstruction effect was the best as well. The Molecular biology results showed that the bone reconstruction was out-sync in each group. Compared with other groups, the bone resorption occurred at the latest and the bone resorption time was the shortest in experimental animals of SCPP group. All results indicated that SCPP could promote osteoblast activity and bone reconstruction, improve the integration of bone interface between prosthesis and base bone, reduce osteoclast activity and shorten the osteoclast action time at the implantation site. Thus, it could postpone or alleviate the occurrence and development of aseptic loosening. Therefore, SCPP could be a promising material for the construction of artificial joints with the ability to resist aseptic loosening.
人工关节无菌性松动是人工关节置换术后最常见的并发症。寻找解决人工关节无菌性松动的方法是骨与关节外科研究领域的重点。我们团队的研究发现，锶掺杂的聚磷酸钙（SCPP）可促进成骨细胞增殖，抑制破骨细胞活性，通过抑制核因子-κ B 配体受体激活剂的表达和提高 OPG 的表达，对无菌性松动具有潜在的抑制作用。本研究旨在通过动物实验来证实这一结论。将涂有 SCPP、聚磷酸钙（CPP）和超高分子量聚乙烯（UHMWPE 的钛棒假体植入股骨（骨隧道内表面也分别涂有 SCPP、CPP 和 UHMWPE）。进行影像学（X 射线、微 CT）、组织化学（苏木精-伊红染色（HE）、亚甲蓝-酸性品红、Von Kossa 组织学染色）、分子生物学（碱性磷酸酶和 TRAP5b 因子、Mir21-5p 和 Mir 26a-5p）分析 SCPP 在 20 周内的作用。影像学结果显示，所有组均发生不同程度的骨溶解，SCPP 组骨溶解最轻微。组织化学结果显示，SCPP 和 CPP 组关节炎较轻，而 SCPP 组骨形成最为显著。其骨重建效果也最好。分子生物学结果表明，各组的骨重建不同步。与其他组相比，SCPP 组的破骨细胞最晚出现骨吸收，骨吸收时间最短。所有结果表明，SCPP 可促进成骨细胞活性和骨重建，改善假体与基底骨之间的骨界面整合，降低破骨细胞活性，缩短植入部位破骨细胞作用时间。因此，它可以推迟或减轻无菌性松动的发生和发展。因此，SCPP 可能是一种有前途的具有抗无菌性松动能力的人工关节构建材料。
