基于催化发夹组装信号放大的 miRNA 检测的传感策略的最新进展。
Recent advances in catalytic hairpin assembly signal amplification-based sensing strategies for microRNA detection.
机构信息
Chongqing Key Laboratory of Inorganic Special Functional Materials, College of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing, 408100, China.
Chongqing Key Laboratory of Inorganic Special Functional Materials, College of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing, 408100, China.
出版信息
Talanta. 2021 Dec 1;235:122735. doi: 10.1016/j.talanta.2021.122735. Epub 2021 Jul 24.
Accumulative evidences have indicated that abnormal expression of microRNAs (miRNAs) is closely associated with many health disorders, making them be regarded as potentialbiomarkers for early clinical diagnosis. Therefore, it is extremely necessary to develop a highly sensitive, specific and reliable approach for miRNA analysis. Catalytic hairpin assembly (CHA) signal amplification is an enzyme-free toehold-mediated strand displacement method, exhibiting significant potential in improving the sensitivity of miRNA detection strategies. In this review, we first describe the potential of miRNAs as disease biomarkers and therapeutics, and summarize the latest advances in CHA signal amplification-based sensing strategies for miRNA monitoring. We describe the characteristics and mechanism of CHA signal amplification and classify the CHA-based miRNA sensing strategies into several categories based on the "signal conversion substance", including fluorophores, enzymes, nanomaterials, and nucleotide sequences. Sensing performance, limit of detection, merits and disadvantages of these miRNA sensing strategies are discussed. Moreover, the current challenges and prospects are also presented.
已有大量证据表明，microRNAs（miRNAs）的异常表达与许多健康障碍密切相关，这使得它们被视为早期临床诊断的潜在生物标志物。因此，开发一种高度敏感、特异和可靠的 miRNA 分析方法是非常必要的。催化发夹组装（CHA）信号放大是一种无酶的、基于引发链置换的方法，在提高 miRNA 检测策略的灵敏度方面具有显著的潜力。在本文中，我们首先描述了 miRNAs 作为疾病生物标志物和治疗药物的潜力，并总结了基于 CHA 信号放大的 miRNA 监测传感策略的最新进展。我们描述了 CHA 信号放大的特点和机制，并根据“信号转换物质”将基于 CHA 的 miRNA 传感策略分为几类，包括荧光团、酶、纳米材料和核苷酸序列。讨论了这些 miRNA 传感策略的传感性能、检测限、优缺点。此外，还提出了当前的挑战和展望。
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