胸腺中T细胞受体γ链表达的分析。

Analysis of T-cell receptor gamma chain expression in the thymus.

作者信息

Pardoll D M, Lew A M, Maloy W L, Fowlkes B J, Kruisbeek A, Bluestone J A, Schwartz R H, Coligan J E

机构信息

Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

出版信息

Adv Exp Med Biol. 1987;225:241-6. doi: 10.1007/978-1-4684-5442-0_21.

DOI:10.1007/978-1-4684-5442-0_21

PMID:3453602

Abstract

While much is known about the structure and function of the T-cell receptor (TCR) alpha beta heterodimer, information has just begun to emerge about the gamma protein, the product of a third rearranging T-cell receptor gene. We describe the use of antiserum to a carboxy-terminal peptide common to several of the murine gamma chain constant regions and a monoclonal antibody to the murine T3 complex to identify products of this T-cell receptor gene family in a subpopulation of Lyt2-, L3T4- thymocytes. The gamma chain has a molecular weight of 35kD, is disulfide bonded to a 45kD partner termed delta (delta) and is associated with the T3 complex. The cells that bear this second T-cell receptor appear to represent a distinct lineage differentiating within the thymus.

摘要

虽然人们对T细胞受体（TCR）αβ异二聚体的结构和功能已了解很多，但关于γ蛋白（第三个重排的T细胞受体基因的产物）的信息才刚刚开始出现。我们描述了使用针对几种小鼠γ链恒定区共有的羧基末端肽的抗血清和针对小鼠T3复合物的单克隆抗体，来鉴定Lyt2-、L3T4-胸腺细胞亚群中这个T细胞受体基因家族的产物。γ链的分子量为35kD，通过二硫键与一个称为δ（delta）的45kD伴侣相连，并与T3复合物相关联。带有这种第二种T细胞受体的细胞似乎代表了在胸腺内分化的一个独特谱系。