生物模型对西尼罗河病毒分离株致病特性形成的影响。
Influence of Biological Model on the Formation of the Pathogenic Properties of the West Nile Virus Isolate.
作者信息
Molchanova E V, Negodenko A O, Luchinin D N, Prilepskaya D R, Khabarova I A, Boroday N V, Baturin A A, Andrianov B V
机构信息
Volgograd Plague Control Research Institute, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Volgograd Region, Russia.
N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow Region, Russia.
出版信息
Bull Exp Biol Med. 2021 Aug;171(4):513-516. doi: 10.1007/s10517-021-05262-9. Epub 2021 Sep 20.
Various biological models are used to isolate West Nile virus, but their role as a selection factor that facilitates selection of isolates with certain properties is usually not evaluated. We compared pathogenic properties of three strains of the West Nile virus obtained from one sample of virus-containing material using different models: WNV Volgograd 900m/18 (on the model of suckling mice), WNV Volgograd 900a/18 (on C6/36 cells) and WNV Volgograd 900v/18 (on Vero cells). WNV Volgograd 900m/18 strain demonstrated virulent (LD 5×10±0.005×10 PFU, p≤0.05) and neuroinvasive properties, induced viremia and pathomorphological changes in the liver, lymph nodes, and brain of nonlinear white mice. WNV Volgograd 900v/18 strain had similar characteristics except for neuroinvasiveness. WNV Volgograd 900a/18 variant demonstrated minimum virulence (LD 5×10±0.005×10 PFU, p≤0.05), did not cause neurological symptoms, and was not isolated from the blood of infected animals.
人们使用各种生物学模型来分离西尼罗河病毒，但通常不会评估它们作为促进具有某些特性的分离株选择的选择因素所起的作用。我们使用不同模型比较了从一份含病毒材料样本中获得的三株西尼罗河病毒的致病特性：伏尔加格勒900m/18株西尼罗河病毒（采用乳鼠模型）、伏尔加格勒900a/18株西尼罗河病毒（采用C6/36细胞）和伏尔加格勒900v/18株西尼罗河病毒（采用Vero细胞）。伏尔加格勒900m/18株西尼罗河病毒表现出强毒力（半数致死剂量为5×10±0.005×10 PFU，p≤0.05）和神经侵袭特性，可引起非转基因小白鼠出现病毒血症以及肝脏、淋巴结和脑部的病理形态学变化。伏尔加格勒900v/18株西尼罗河病毒除神经侵袭性外具有相似特征。伏尔加格勒900a/18变种表现出最低毒力（半数致死剂量为5×10±0.005×10 PFU，p≤0.05），未引起神经症状，且未从感染动物血液中分离到。
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