亚铁（Fe）对硫驱动自养反硝化的抑制作用：对微生物群落和功能基因的洞察
Inhibition of ferrous iron (Fe) to sulfur-driven autotrophic denitrification: Insight into microbial community and functional genes.
作者信息
Pang Yunmeng, Wang Jianlong
机构信息
Laboratory of Environmental Technology, INET, Tsinghua University, Beijing 100084, PR China.
Laboratory of Environmental Technology, INET, Tsinghua University, Beijing 100084, PR China; Beijing Key Laboratory of Radioactive Waste Treatment, INET, Tsinghua University, Beijing 100084, PR China.
出版信息
Bioresour Technol. 2021 Dec;342:125960. doi: 10.1016/j.biortech.2021.125960. Epub 2021 Sep 20.
The effect of Fe on the performance of sulfur-driven autotrophic denitrification (SDAD) using S as electron donor was evaluated. The experimental results showed that as initial Fe concentration increased, nitrate (NO) removal rate significantly decreased. Fe ion (0.1 mM and 1 Mm) inhibited SDAD rate (approximately 10% and 50%) and resulted in an accumulation of nitrite (NO) and nitrous oxide (NO). The relative abundance of Thiobacillus was positively correlated with NO removal rate, whereas negatively correlated with Fe concentration, suggesting that Fe inhibited the sulfur-oxidizing denitrifying bacteria. Moreover, the abundance of bacterial 16S rRNA, denitrifying genes (narG, nirS, nirK and nosZ) and sulfur-oxidizing genes (soxB and dsrA) decreased with the increase of Fe concentration, among them nosZ and soxB were the most sensitive genes to Fe, and nosZ/narG, soxB/(bacterial 16S rRNA) and soxB/nirK had influence on NO removal rate, while nosZ/(bacterial 16S rRNA) affected NO accumulation rate.
评估了铁对以硫为电子供体的硫驱动自养反硝化（SDAD）性能的影响。实验结果表明，随着初始铁浓度的增加，硝酸盐（NO）去除率显著降低。铁离子（0.1 mM和1 mM）抑制了SDAD速率（分别约为10%和50%），并导致亚硝酸盐（NO₂）和一氧化二氮（N₂O）的积累。硫杆菌的相对丰度与NO去除率呈正相关，而与铁浓度呈负相关，这表明铁抑制了硫氧化反硝化细菌。此外，细菌16S rRNA、反硝化基因（narG、nirS、nirK和nosZ）和硫氧化基因（soxB和dsrA）的丰度随着铁浓度的增加而降低，其中nosZ和soxB是对铁最敏感的基因，并且nosZ/narG、soxB/（细菌16S rRNA）和soxB/nirK对NO去除率有影响，而nosZ/（细菌16S rRNA）影响NO积累率。
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